Research Methods | National Institute of Environmental Health Sciences
Source: https://www.niehs.nih.gov/research/atniehs/labs/iidl/host-def/methods
Archived: 2026-04-23 17:18
Research Methods | National Institute of Environmental Health Sciences
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Clinical Investigation of Host Defense Group
In vivo murine models of disease:
Lung fibrosis – bleomycin, silica, BHT
Lung inflammation – LPS, cigarette smoke
Pneumonia – bacteria, virus
Asthma – House dust mite, OVA
Systemic infection –
L. monocytogenes
Multiple sclerosis – EAE
Cancer – B16F10 melanoma, MC38 adenocarcinoma
In vivo
methods – adoptive cell transfer, bone marrow chimeras, lineage tracing
In vitro/ex vivo
molecular and cell biology methods:
Cell culture
Stable cell lines, siRNA/shRNA/CRISPR-CAS9
Biochemistry – immunoblotting, immunoprecipitation
scRNA-Seq, ATAC-Seq, ChIP-Seq
Seahorse
Metabolomic, proteomic analysis
Confocal microscopy, super-resolution microscopy
Flow cytometry, FACS
Isolation and stimulation of human peripheral blood leukocytes
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to Top
Last Reviewed: April 10, 2026
Skip Navigation
Research Methods
Close the left navigation
Add
Clinical Investigation of Host Defense Group
In vivo murine models of disease:
Lung fibrosis – bleomycin, silica, BHT
Lung inflammation – LPS, cigarette smoke
Pneumonia – bacteria, virus
Asthma – House dust mite, OVA
Systemic infection –
L. monocytogenes
Multiple sclerosis – EAE
Cancer – B16F10 melanoma, MC38 adenocarcinoma
In vivo
methods – adoptive cell transfer, bone marrow chimeras, lineage tracing
In vitro/ex vivo
molecular and cell biology methods:
Cell culture
Stable cell lines, siRNA/shRNA/CRISPR-CAS9
Biochemistry – immunoblotting, immunoprecipitation
scRNA-Seq, ATAC-Seq, ChIP-Seq
Seahorse
Metabolomic, proteomic analysis
Confocal microscopy, super-resolution microscopy
Flow cytometry, FACS
Isolation and stimulation of human peripheral blood leukocytes
Back
to Top
Last Reviewed: April 10, 2026