Isoform Usage Two-step Analysis (IUTA) | National Institute of Environmental Health Sciences Skip Navigation Isoform Usage Two-step Analysis (IUTA) Close the left navigation Add About IUTA IUTA is an analysis tool of Illumina paired-end mRNA-seq data for detecting differential usage of gene transcript isoforms. IUTA stands for the soform sage wo-step nalysis. It first uses the EM algorithm to identify the usage of transcript isoforms for each gene, then tests the difference in the isoform usage between two groups based on the method for composition data analysis. Tests on both simulated and read mRNA-seq data showed IUTA can accurately estimate isoform usage and detect usage change between two groups. IUTA is available as an R package freely to the public. IUTA R Packages IUTA source R package: IUTA_1.0-sourcecode.tar.gz (814KB) IUTA binary R package for MacOS X OS: IUTA_1.0-bin-MacOSX.tgz (856KB) IUTA binary R package for Windows OS: IUTA_1.0-bin-Windows.zip (861KB) IUTA PDF manual (162KB) Contact David M. Umbach, Ph.D. Staff Scientist Tel 984-287-3703 [email protected] Leping Li, Ph.D. Principal Investigator Tel 984-287-3836 [email protected] Back to Top Last Reviewed: December 31, 2025