Gene Editing and Mouse Model Core Facility | National Institute of Environmental Health Sciences

Gene Editing and Mouse Model Core Facility | National Institute of Environmental Health Sciences
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Gene Editing and Mouse Model Core Facility
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Background
The
Gene Editing and Mouse Model Core Facility
is primarily tasked with producing genetically modified animals (GMA) for intramural researchers. In addition to GMA, the core collaborates with intramural researchers to develop genetically modified cell lines. In addition to genome editing projects, the core also offers a variety of services associated with research animals such as mouse colony importation and cryo-preservation/recovery of mouse lines. The GEMM Core staff also provides their expertise in troubleshooting issues in either previously made mouse/cell lines or on-going genome editing projects designed by intramural investigators and/or their extramural collaborators.
Services We Provide
Develop genome editing strategy and generate locus specific reagents for genome editing projects
Transfection of mouse embryonic stem cells with targeting vector and subsequent culturing, clonal expansion, and clonal screening
Microinjection of genetically modified ES cells into mouse blastocysts to generate chimeras and their subsequent breeding to identify germline transmission or chimeric analysis
Culturing of user-provided genetically modified ES cells for QAL/QC, and subsequent blastocyst micro-injection
Single-cell embryo micro-injection of CRISPR/Cas9 system to generate targeted genomic mutations
Note: Traditional random integration transgene projects are outsourced
CRISPR/Cas9-mediated genome editing in mouse ES cells and immortalized cell lines
Derivation of mouse ES cell lines from existing genetically modified mouse lines
Mouse embryo cryo-recovery and cryo-preservation
Assist in troubleshooting problems with previously generated genetically modified mouse lines
Long-term storage of frozen mouse embryos
In collaboration with CMB/ACUC, assist intramural investigators in developing efficient breeding strategy to optimize animal use and minimizing the animal cost for the institute
Video
NIEHS Shared and Core Facilities are available to NIH researchers. Information for staff on utilizing these services can be found on the NIEHS Junction or by contacting the staff below.
Scientific Staff
Manas K. Ray, Ph.D.
Co-Director, Gene Editing and Mouse Model Core Facility
Tel 984-287-4312
[email protected]
Artiom Gruzdev, Ph.D.
Co-Director, Gene Editing and Mouse Model Core Facility
Tel 984-287-4258
[email protected]
Gregory J. Scott
Biologist
Tel 984-287-4296
[email protected]
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Last Reviewed: March 04, 2026