Mouse & Rat Embedding Suggestions | National Institute of Environmental Health Sciences

Mouse & Rat Embedding Suggestions | National Institute of Environmental Health Sciences
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Necropsy Support
Much of the work carried out by DTT is in support of the National Toxicology Program (NTP), an interagency partnership of the Food and Drug Administration, National Institute for Occupational Safety and Health, and NIEHS.
Visit the NTP Website
Sampling, Trimming and Embedding Guides
The following interactive guides and images may be helpful to those using rats and mice for regulatory type toxicity studies.
The interactive guides and images were created by David Savio and Beth Mahler. Three guides from RITA and NACAD are for organ sampling and trimming in rats and mice. These guides were originally published in Experimental Toxic Pathology, in 2003, Vol. 55, publisher: URBAN & FISCHER
Ruehl-Fehlert C, Kittel B, Morawietz G, Deslex P, Keenan C, Mahrt CR, Nolte T, Robinson M, Stuart BP, Deschl U; RITA Group; NACAD Group. 2003. Revised guides for organ sampling and trimming in rats and mice – Part 1. Exp Toxicol Pathol 55:91-106. [
Abstract
]
Kittel B, Ruehl-Fehlert C, Morawietz G, Klapwijk J, Elwell MR, Lenz B, O’Sullivan MG, Roth DR, Wadsworth PF; RITA Group; NACAD Group. 2004. Revised guides for organ sampling and trimming in rats and mice – Part 2. Exp Toxicol Pathol 55:413-431. [
Abstract
]
Morawietz G, Ruehl-Fehlert C, Kittel B, Bube A, Keane K, Halm S, Heuser A, Hellmann J; RITA Group; NACAD Group. 2004. Revised guides for organ sampling and trimming in rats and mice – Part 3. Exp Toxicol Pathol 55:433-449. [
Abstract
]
Mouse Embedding Suggestions
Adrenal Glands
Brain L1-L4
Brain L5-L7
Clitoral Glands
Eye 01
Eye 02
Femur
Harderian Glands
Heart Aorta Tongue
Ileum Cecum Colon Rectum
Kidney
Larynx
Liver
Lungs
Mesenteric Mandibular Bronchial Lymph Nodes
Nasal Turbinates L1-L3
Ovaries
Pancreas
Pituitary
Preputial Glands
Prostate Sem Ves Coag Gland Bladder
Salivary Glands
Skeletal Muscle Skeletal Muscle Nerve
Skin Mammary
Spinal Cord
Spleen Thymus
Stomach Duodenum Jejunum
Testes Epididymis
Thyroid Parathyroid Esophagus Trachea
Uterus
Vagina Uterine Horn Cross Section Bladder
Zymbals
Rat Embedding Suggestions
Adrenals
Bladder
Brain L1 Olfactory Bulbs
Brain L2-L4
Brain L5-L7
Clitoral Gland
Epididymis
Eye 01
Eye 02
Femur
Harderian Glands
Heart Aorta Tongue
Ileum Cecum Colon Rectum
Kidney
Larynx
Liver
Lung Apical Cardiac
Lung Diaphragmatic Accessory
Lung Left Lobe
Mesenteric Mandibular Bronchial Lymph
Nasal Turbinates L1-L2
Nasal Turbinates L3
Ovaries
Pancreas
Pituitary
Preputial Glands
Prostate Sem Ves Coag Gland
Skeletal Muscle Nerve
Skin Mammary
Spinal Cord
Spleen Thymus
Stomach Duodenum Jejunum
Testes
Thyroid Parathyroid Esophagus Trachea
Uterus
Vagina Uterine Horn Cross Section
Zymbals
Related Links
Pathology Support Group - Necropsy Support
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Last Reviewed: January 05, 2026