Immunohistochemistry | National Institute of Environmental Health Sciences
Source: https://www.niehs.nih.gov/research/atniehs/labs/cmp/path-support/immuno
Archived: 2026-04-23 17:11
Immunohistochemistry | National Institute of Environmental Health Sciences
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Immunohistochemistry
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Much of the work carried out by DTT is in support of the National Toxicology Program (NTP), an interagency partnership of the Food and Drug Administration, National Institute for Occupational Safety and Health, and NIEHS.
Visit the NTP Website
Pathology Support Group
The Immunohistochemistry Support Team performs immunohistochemical stains on tissues and cells for NIEHS Division of Intramural Research and Division of Translational Toxicology researchers.
Immunohistochemistry Support Staff
Renee D. Fisher
Supervisory Biologist
Tel 984-287-3956
[email protected]
Heather L. Jensen, M.S.
Lead Biologist
Tel 984-287-3930
[email protected]
Mark A. Rubino
Biologist
Tel 984-287-3934
[email protected]
Charan K. Ganta, B.V.Sc., Ph.D., D.A.C.V.P.
Contractor - IHC Pathologist
Tel 984-287-3833
[email protected]
David J. Olson
Contractor
Tel 984-287-3933
[email protected]
Tara W. Moulden
Contractor
Tel 984-287-3932
[email protected]
Capabilities
Highly trained staff members provide the following key functions:
Develop staining protocols for tissue preserved in a variety of fixatives.
10% neutral buffered formalin, 4% paraformaldehyde, ethanol, methanol.
Enzyme based visualization using brown, red, blue, and purple chromogenic substrates.
Offer antibody selection assistance.
Perform co-localization experiments using chromogen or fluorescent detection.
Perform cryopreservation and sectioning of frozen tissue samples.
Perform immunohistochemical stains on formalin-fixed paraffin-embedded tissue or OCT embedded frozen tissue sections and cytology samples.
Perform Immunofluorescence on paraffin-embedded or frozen tissue sections.
Perform single or dual localization.
Provide information on tissue handling and preparation that promote optimal staining results.
Provide pathology reports.
Provide routine antibodies for IHC staining.
Work closely with NIEHS researchers to develop and optimize staining procedures for novel and commercially available antibodies.
Routine Antibodies provided by the Immunohistochemistry (IHC) Laboratory
beta catenin
BrdU
caspase-3
CD3
ED2
F4/80
Factor VIII
Iba1
Ki67
p53
pancytokeratin
Pax-5
PCNA
TUNEL
vimentin
Related Links
Immunohistochemistry in Investigative and Toxicologic Pathology
Immunohistochemistry Protocols
IRGM1 Supports Host Defense Against Intracellular Bacteria Through Suppression of Type I Interferon in Mice
(598KB)
Neurophysiological Assessment if Peripheral Nerve, Somatosensory, and Brainstem Auditory Function after Perinatal Exposure to Emamectin Long Evan Rats
(3MB)
Reagents
Retrievals
Back
to Top
Last Reviewed: February 18, 2026
Skip Navigation
Immunohistochemistry
Close the left navigation
Add
Much of the work carried out by DTT is in support of the National Toxicology Program (NTP), an interagency partnership of the Food and Drug Administration, National Institute for Occupational Safety and Health, and NIEHS.
Visit the NTP Website
Pathology Support Group
The Immunohistochemistry Support Team performs immunohistochemical stains on tissues and cells for NIEHS Division of Intramural Research and Division of Translational Toxicology researchers.
Immunohistochemistry Support Staff
Renee D. Fisher
Supervisory Biologist
Tel 984-287-3956
[email protected]
Heather L. Jensen, M.S.
Lead Biologist
Tel 984-287-3930
[email protected]
Mark A. Rubino
Biologist
Tel 984-287-3934
[email protected]
Charan K. Ganta, B.V.Sc., Ph.D., D.A.C.V.P.
Contractor - IHC Pathologist
Tel 984-287-3833
[email protected]
David J. Olson
Contractor
Tel 984-287-3933
[email protected]
Tara W. Moulden
Contractor
Tel 984-287-3932
[email protected]
Capabilities
Highly trained staff members provide the following key functions:
Develop staining protocols for tissue preserved in a variety of fixatives.
10% neutral buffered formalin, 4% paraformaldehyde, ethanol, methanol.
Enzyme based visualization using brown, red, blue, and purple chromogenic substrates.
Offer antibody selection assistance.
Perform co-localization experiments using chromogen or fluorescent detection.
Perform cryopreservation and sectioning of frozen tissue samples.
Perform immunohistochemical stains on formalin-fixed paraffin-embedded tissue or OCT embedded frozen tissue sections and cytology samples.
Perform Immunofluorescence on paraffin-embedded or frozen tissue sections.
Perform single or dual localization.
Provide information on tissue handling and preparation that promote optimal staining results.
Provide pathology reports.
Provide routine antibodies for IHC staining.
Work closely with NIEHS researchers to develop and optimize staining procedures for novel and commercially available antibodies.
Routine Antibodies provided by the Immunohistochemistry (IHC) Laboratory
beta catenin
BrdU
caspase-3
CD3
ED2
F4/80
Factor VIII
Iba1
Ki67
p53
pancytokeratin
Pax-5
PCNA
TUNEL
vimentin
Related Links
Immunohistochemistry in Investigative and Toxicologic Pathology
Immunohistochemistry Protocols
IRGM1 Supports Host Defense Against Intracellular Bacteria Through Suppression of Type I Interferon in Mice
(598KB)
Neurophysiological Assessment if Peripheral Nerve, Somatosensory, and Brainstem Auditory Function after Perinatal Exposure to Emamectin Long Evan Rats
(3MB)
Reagents
Retrievals
Back
to Top
Last Reviewed: February 18, 2026