AAU

AAU
Central Instrumentation Facility Laboratory
About Us
Central Instrumentation Facility (CIF) Laboratory
The premier institute of North East India, Assam Agricultural University was established on April 1,
1969 under The Assam Agricultural University Act, 1968, with the mandate of imparting farm
education, conduct research in agriculture and allied sciences, and to effectively disseminate
technologies so generated. One of the mandates of AAU is to undertake basic, strategic and applied
research in the sector for agriculture centric business and employment avenues in the production to
consumption chain. The University is the recipient of prestigious “Sardar Patel Outstanding
Instituion Award, 2014”.
Central Instrumentation Facility (CIF) laboratory at Assam
Agricultural
University, Jorhat was established in the year 2006 as a separate unit in the Department of
Bio-chemistry and
Agricultural Chemistry. The aim of establishing this laboratory was to provide
central instrumentation facilities to those M.Sc./Ph.D. students who didn’t have such facilities
in their respective Department. In August, 2019 this lab was shifted to DBT-AAU Centre/Department of
Agricultural Biotechnology under the supervision of Professor Bidyut Kumar Sarmah, Director of
DBT-NECAB.
So far, many students and faculties of both AAU and outside AAU were
benefitted by
the facility of the CIFL during their post graduation research. Moreover, CIFL helps the farming
community/ budding entrepreneurs to analyze their products for microbial contamination and /or
various biochemical components.
Recently, the vision of the laboratory has been changed to make it
more active and
accessible for other public and private institutes. This laboratory basically targets the
biochemical, microbial and biotechnological analysis on payment basis so that it generates revenue
for its long term sustainability. Along with analysis, there is short term training facility for the
students pursuing B. Sc. / M. Sc. dgree programme and labroratory technicians of other
colleges/universities.
Mandate
Helping students/researchers /faculties of Assam Agricultural University(AAU) and outside
AAU in
their research.
To offer hands on training to students/researchers /faculties of Assam Agricultural
University (AAU)
and outside AAU. Also to offer hands on training to food entrepreneurs.
National Accreditation Board for Testing and Calibration Laboratories
Certificate of Accreditation
Scope of Accreditation
Presently we offer following facilities
Analysis of phytochemicals (total phenol, chlorophylls, total flavonoids, total anthocyanin,
beta- carotene, curcumin, trypsin inhibitor, caffeine, ascorbic acid, etc.)
Proximate composition analysis of food/plants
Microbial analysis of food/plants
Molecular biology related analysis (DNA/RNA isolation, PCR
amplification, Gel running, etc.)
Short term training on biochemical, microbiological and molecular
techniques
Some important parameters studied in CIFL on payment basis
Estimation of nitrogen by micro-kjeldahl method
Estimation of non-protein nitrogen
Estimation of protein nitrogen
Estimation of proline
Estimation of methionin
Estimation of tryptophan
Estimation of Lysine
Estimation of protein by Lowery's method
Estimation of reducing and non-reducing sugars
Estimation of total soluble sugars
Estimation of amylose
Estimation of amylopectin
Estimation of starch
Estimation of glycogen
Estimation of cellulose/hemi-cellulose
Estimation of crude fibre
Estimation of pectic substances
Estimation of total lipids
Estimation of oil/crude fat in oilseed
Estimation of free fatty acid or acid value of an oil
Estimation of peroxide value of an oil
Estimation of saponification value of oil
Estimation of ascorbic acid(vitamin-C)
Estimation of riboflavin
Estimation of ash content
Estimation of phosphorus
Estimation of total phenol
Estimation of chlorophyll
Estimation of carbohydrate
Estimation of trypsin inhibitor
Estimation of phytic acid
Estimation of dry matter and moisture
Estimation of Nitrate content
Estimation of oxalate
Protein electrophoresis:
Native PAGE
SDS PAGE
DNA/RNA isolation*
PCR*
16sRNA amplification
ITS amplification
Some important instruments for using on payment basis
Spectrophotometer
Laminar Air flow
Autoclave
pH meter
Centrifuge
BOD Incubator
Gel Doc
Candidates should apply through proper channel to Dr B.K.Sarmah, In Charge,
CIFL and Director, DBTNECAB, AAU, Jorhat:785013, email : bidyut.sarmah[at]aau.ac.in
For payment communicate the following persons
Mrs. Malobika Borkoch (malobika.borkoch.abt[at]aau.ac.in)
Dr. Tankeswar Nath (tankeswar.nath[at]aau.ac.in)
Short term training
A short term hands-on-training (10days) will be conducted on Biochemical, Microbiological and
Molecular techniques by the Central Instrumentation Facility Laboratory (CIFL), Assam Agricultural
University, Jorhat for the students of general colleges of Jorhat and neibouring districts.
Information regarding future training will be announced in due time
Subject-wise specific Training
Biochemical analysis
: Moisture content, Protein, Total Carbohydrate, Reducing Sugar, Non
reducing sugar, Crude fat, Crude fiber, Total ash, etc.
Microbiological analysis
: Isolation of microbes, purification of microbes, Identification
of
microbes (based on morphological and biochemical parameters)
Molecular biology related analysis
: DNA extraction and purification, Amplification
through PCR,
Electrophoresis of amplified DNA
Modules and Team Members
Module
Team Members
Biochemical analysis
Mrs Malobika Borkoch
Dr Sunayana Rathi
Dr. Tankeswar Nath
Dr. Priyanka Das
Microbiological analysis
Mrs Malobika Borkoch
Dr Robin Chandra Boro
Dr. Tankeswar Nath
Dr. Madhumita Barooah
Molecular biology related analysis
Mrs Malobika Borkoch
Dr. Sumita Acharjee
Dr. Priyabrata Sen