Cytometry – UF ICBR
Cytometry
ICBR-Cytometry@ad.ufl.edu
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(352) 273-6032
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RRID:SCR_019119
Cytometers, Confocal Microscopes, Histology, In-Vivo Imaging & More
CORE OVERVIEW
The ICBR Cytometry core provides investigators with access to Cytometry and Optical Microscopy instrumentation and services. We offer project consulting, with experimental design and optimization to help researchers plan both large and small-scale experiments utilizing our cell sorters, analyzers, small particle instruments and microscopes. Training is offered for most of our instruments with an eye towards creating knowledgeable, self-sufficient operators. We coordinate closely with other ICBR cores (Next-Gen Sequencing, Gene Expression, Bioinformatics, Monoclonal Antibody, Electron Microscopy, and Proteomics) on shared projects. Our core offers a large suite of services including staff-assisted instrument operation, cytometry theory and hands-on training courses, data analysis workstations, online analysis software licenses, and 24/7 self-service access to most of our equipment. Our cytometers, small particle instruments and microscopes are distributed between two buildings on the University of Florida’s Health Science Center campus, the Cancer Genetics Research Complex (CGRC) and the McKnight Brain Institute (MBI).
The Cytometry core provides a variety of tools and staff expertise for analyzing and imaging live cells, fixed cells, tissue sections, and small particles (i.e.: like EVs, exosomes, liposomes). The laboratory incorporates numerous flow cytometers, from simple entry-level analyzers to high-end 5-laser, 28-parameter instruments to analyze and sort cells. We have sorters to help users collect cells of interest: BD Symphony S6, BD Fusion, and a Sony SH800. Our collection of analyzing cytometers includes several traditional filter-based systems (Cytoflex LX, Fortessa, Accuri, Canto, Symphony A3) as well as spectral analyzers (Sony ID7000 and Cytek Aurora). For those who are working with small particles, we also have a NanoFCM and a NanoSight NS300.
In addition, the core maintains an assortment of microscopes, in both confocal and widefield formats. The microscopes are well equipped to work with a variety of fluorescent labels from near UV out to long-red. Within in our assortment of imaging capabilities are a number of advanced functions, including FLIM, FRET, FRAP, image stitching, 3D processing, and in-vitro time-lapse imaging. We offer a range of microscope models including our White-Light Laser equipped Leica Stellaris 8 spectral confocal, a Leica SP5 spectral confocal, and two Olympus IX81-DSU spinning disk confocals. Our collection of widefield microscopes includes a Keyence BZ-X800, and a Nikon Ti-E equipped for live-cell imaging.
CORE NEWS
ICBR Closed Friday (4/10) for Team Building
ICBR Closed Friday (4/10) for Team Building
ICBR Closed Friday, 11:00am-5:00pm for a Team Building Activity
ICBR will be closed from 11am-5pm on Friday for a special team building event. We will not be accepting packages or samples after 11am on Friday. Our normal business hours will resume on Monday.
Please refer to our staff directory for additional details.
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Covid-19 Vaccines Enhance the Effectiveness of Immune Checkpoint Inhibitors in Fighting Cancer
Covid-19 Vaccines Enhance the Effectiveness of Immune Checkpoint Inhibitors in Fighting Cancer
Covid-19 Vaccines Enhance the Effectiveness of Immune Checkpoint Inhibitors in Fighting Cancer
Steven J. Madore, PhD (ICBR Associate Director for Science, UFHCI Associate Director for Shared Resources)
Tumor cells fend off the attacks of immune system T-cells by expressing proteins like PD-L1 on their cell surface. The PD-L1 on the surface of the tumor cell binds to a T-cell surface receptor protein called PD-1 and silences the killing action of the T-cells. Blocking the binding of PD-L1 to PD...
ICBR Closed for Homecoming, Friday, October 17th
ICBR Closed for Homecoming, Friday, October 17th
UF | ICBR will be closed on Friday, October 17th in observance of UF Homecoming. Please enjoy the long weekend! Normal business hours will resume on Monday, October 20th.
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ICBR Closed Monday for Labor Day
ICBR Closed Monday for Labor Day
ICBR Closed Monday for Labor Day
ICBR will be closed this Monday (9/1) in observation of Labor Day
We will not be accepting packages or samples on Monday. Our normal business hours will resume on Tuesday (9/2). Enjoy the the long weekend!
Please refer to our staff directory for additional details.
...
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
by Joe Kays
University of Florida plant molecular biologist Anna-Lisa Paul has been named a recipient of the Southeastern Universities Research Association’s (SURA) 2025 Distinguished Scientist Award, recognizing her groundbreaking contributions to space biology and her leadership in advancing scientific exploration beyond Earth.
Paul is the director of UF’s Interdisciplinary Center for Biotechnology Research (ICBR),...
Environmental DNA – It’s All Up in the Air
Environmental DNA – It’s All Up in the Air
Environmental DNA – It’s All Up in the Air
Steven J. Madore, PhD (ICBR Associate Director for Science)
(Note: this article has been summarized from UF News “Environmental DNA floating in the air tracks wildlife, viruses — even drugs” June 3, 2025 by Eric Hamilton)
Scientists can now use the power of genetic information to identify biodiversity by sampling environmental DNA or eDNA.
“The level of information that’s available in environmental DNA is such that we’re only starting to consider what t...
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
Steven J Madore, PhD (ICBR Associate Director for Science)
Summarized from J. Clin. Inv. 2025;135(6); e185149
Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide. Despite a combination of surgery and chemotherapy, survival rate remains low due to cancer cell metastasis and drug resistance. Mutations in the oncogene KRAS are frequently found in human cancers, including up to ...
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
The U.S. National Science Foundation National Radio Astronomy Observatory (NSF NRAO) and the University of Florida (UF) have announced a dynamic new partnership to advance science education and public outreach at a future Next Generation Very Large Array (ngVLA) antenna site in Florida.
This collaboration, signed with a Memorandum of Understanding (MOU) at the debut of the ngVLA prototype antenna in New Mexico l...
CORE INSTRUMENTS
Cytometry
BD Accuri C6
CytoFLEX Flow Cytometer
Cytek Aurora
Leica TCS SP5 Confocal
Leica Stellaris 8/STED System
BD FACSSymphony S6 Sorter
Olympus IX81-DSU
NanoFCM
BD FACSAria Fusion
Malvern NanoSight NS300
BD FACSymphony A3
Zeiss Confocal LSM800 Microscope
Cytometry
Contact & Personnel
Services
Instruments
Core Resources
Instrument Resources
Acknowledge Core
Proper Citation:
University of Florida ICBR Cytometry Core Facility, RRID:SCR_019119
What’s an RRID?
Contact
ICBR-Cytometry@ad.ufl.edu
(352) 273-6032
Locations
Cancer & Genetics Research Complex
Room 292 CGRC
2033 Mowry Road
Gainesville FL 32610
McKnight Brain Institute
Room L3-160 MBI
1149 Newell Drive
Gainesville FL 32610