…ncubated overnight (4°C) with primary antibodies including FAM20C (Proteintech, 25395-I-AP), p-AKT Ser473 (Cell Signaling, 9271S), AKT (Cell Signaling, 9272S), CNPY4 (AF5015, R&D Systems), and Actin-HRP (Life Technologies, MA515739HRP). Detection of proteins was carried out by in…