Gene Expression & Genotyping – UF ICBR

Gene Expression & Genotyping – UF ICBR
Gene Expression & Genotyping
ICBR-Genotyping@ad.ufl.edu
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ICBR-GeneExpression@ad.ufl.edu
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(352) 273-8043
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RRID:SCR_019145
Single Cell RNA-Seq, CRISPR Services, Digital PCR and More
CORE OVERVIEW
Welcome to UF ICBR Gene Expression and Genotyping Core (GE). Our up-to-date gene expression and genotyping analysis methods allow scientists to be on the cutting edge of research. Our high-quality data can help researchers for their grant applications and scientific paper publications.
The Gene Expression & Genotyping core provides state-of-the-art technical services and consultation on
Single Cell RNA-Seq, 10x Visium Spatial Gene Expression, Covid-seq
RNA-Seq
16s metagenomics
and ThemoFisher
Axiom Genotyping arrays
using Beckman Biomek Liquid Handling Automation instrument. High-throughput RNA-seq libraries, 16s libraries and sequencing capture libraries can be done by using the SPT Labtech nanoliter pipetting instrument Mosquito, DragonFly and Agilent Bravo robot.
QuantiGene RNA Assa
ys measure up to 80 gene targets directly with degraded and cross-linked RNA in FFPE tissues and blood, with no RNA purification required. The BioRad QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules with greater precision and sensitivity than qPCR, the sensitivity off
ddPCR
System can facilitate expanded analysis of single cells.
To help researchers at every aspect, GE also provides a variety of basic molecular biology services such as DNA isolations, PCR reactions, and PCR purifications for sequencing. High quality of DNA and RNA are the key for reliable data; therefore, DNA and RNA quality assessment services are offered using an Agilent Bioanalyzer and TapeStation instrument at GE.
The walkup service for DNA RNA extraction with QIAcube is available at GE as well, for more information, please contact us at
GeneExpression@ad.ufl.edu
SERVICES OVERVIEW
General Services
Library Construction Services
Single Cell Analysis
Genotyping Services
Project Consultation
Digital PCR services
RNA DNA QC: Bioanalyzer/TapeStation, Qubit, PicoGreen
CRISPR services
Automated NextGen Library Preparation
QuantiGen assays (up to 80 targets): Gene Expression and CNV
16s metagenomics library Automation
Illumina Directional RNA-Seq Library Preparation (Poly A)
Illumina Non-Directional RNA-Seq Library Preparation (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina Small RNA libray w/Barcode
RNA-Seq library automation
rRNAs Depletion
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with/without Pre-pooling
Takara Single Cell Library Construction
16s library Automation Per Sample (batch w/48 samples, 96 samples)
Illumina RNA-Seq libraries (Poly A)
Illumina RNA-Seq library Prep for low input, ribo-depletion or degraded RNA
Illumina RNA-Seq library Prep (polyA with 140 samples and up, 500 samples and up)
Illumina Small RNA-Seq library w/Barcode
NEB Directional RNA-Seq Library Preparation
NEB Non-directional RNA-Seq Library preparation
NextGen Sequencing Library Quantification
RNA-Seq library automation per sample (24 samples, 48 samples, 96 samples)
rRNAs Depletion
RNA Extraction, RNA Clean-up
Agilent SureSelect Target Enrichment
IDT Exome Capture Library Construction with Pre-pooling, without Pre-pooling
10X Genomics Chromium 3’ Single Cell RNA-Seq
10X Genomics Chromium 5’ Single Cell RNA-Seq
10X Genomics Chromium Single Cell CNV analysis
10X Genomics Single Human/Mouse T cell V(D)J Library Prep (per sample)
10X Genomics Chromium Single Cell Feature Barcode for CITE-Seq
10X Genomics Chromium Single Cell Feature Barcode for CRISPR Screening
SNP analysis by qPCR or digital PCR
Looking for data analysis services?
Visit
ICBR Bioinformatics
for services.
Don’t see the service you are looking for?
Visit
ICBR NextGen DNA Sequencing
for more options.
CORE NEWS
ICBR Closed Friday (4/10) for Team Building
ICBR Closed Friday (4/10) for Team Building
ICBR Closed Friday, 11:00am-5:00pm for a Team Building Activity
ICBR will be closed from 11am-5pm on Friday for a special team building event. We will not be accepting packages or samples after 11am on Friday. Our normal business hours will resume on Monday.
Please refer to our staff directory for additional details.
...
Covid-19 Vaccines Enhance the Effectiveness of Immune Checkpoint Inhibitors in Fighting Cancer
Covid-19 Vaccines Enhance the Effectiveness of Immune Checkpoint Inhibitors in Fighting Cancer
Covid-19 Vaccines Enhance the Effectiveness of Immune Checkpoint Inhibitors in Fighting Cancer
Steven J. Madore, PhD (ICBR Associate Director for Science, UFHCI Associate Director for Shared Resources)

Tumor cells fend off the attacks of immune system T-cells by expressing proteins like PD-L1 on their cell surface.  The PD-L1 on the surface of the tumor cell binds to a T-cell surface receptor protein called PD-1 and silences the killing action of the T-cells. Blocking the binding of PD-L1 to PD...
ICBR Closed for Homecoming, Friday, October 17th
ICBR Closed for Homecoming, Friday, October 17th
UF | ICBR will be closed on Friday, October 17th in observance of UF Homecoming. Please enjoy the long weekend! Normal business hours will resume on Monday, October 20th.
...
ICBR Closed Monday for Labor Day
ICBR Closed Monday for Labor Day
ICBR Closed Monday for Labor Day
ICBR will be closed this Monday (9/1) in observation of Labor Day

We will not be accepting packages or samples on Monday. Our normal business hours will resume on Tuesday (9/2). Enjoy the the long weekend!

Please refer to our staff directory for additional details.
...
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
UF plant biologist Anna-Lisa Paul receives SURA’s Distinguished Scientist Award
by Joe Kays
University of Florida plant molecular biologist Anna-Lisa Paul has been named a recipient of the Southeastern Universities Research Association’s (SURA) 2025 Distinguished Scientist Award, recognizing her groundbreaking contributions to space biology and her leadership in advancing scientific exploration beyond Earth.
Paul is the director of UF’s Interdisciplinary Center for Biotechnology Research (ICBR),...
Environmental DNA – It’s All Up in the Air
Environmental DNA – It’s All Up in the Air
Environmental DNA – It’s All Up in the Air
Steven J. Madore, PhD (ICBR Associate Director for Science)
(Note: this article has been summarized from UF News “Environmental DNA floating in the air tracks wildlife, viruses — even drugs” June 3, 2025 by Eric Hamilton)
Scientists can now use the power of genetic information to identify biodiversity by sampling environmental DNA or eDNA.
“The level of information that’s available in environmental DNA is such that we’re only starting to consider what t...
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
UF Investigators Identify Mechanism of Drug Resistance in Non-Small Cell Lung Cancer
Steven J Madore, PhD (ICBR Associate Director for Science)
Summarized from J. Clin. Inv. 2025;135(6); e185149
Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths worldwide. Despite a combination of surgery and chemotherapy, survival rate remains low due to cancer cell metastasis and drug resistance.  Mutations in the oncogene KRAS are frequently found in human cancers, including up to ...
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
Stars Align for Science: University of Florida and NSF NRAO Forge Path for Discovery
The U.S. National Science Foundation National Radio Astronomy Observatory (NSF NRAO) and the University of Florida (UF) have announced a dynamic new partnership to advance science education and public outreach at a future Next Generation Very Large Array (ngVLA) antenna site in Florida.
This collaboration, signed with a Memorandum of Understanding (MOU) at the debut of the ngVLA prototype antenna in New Mexico l...
CORE INSTRUMENTS
Gene Expression & Genotyping
BioRad QX200 Digital PCR System
BD Rhapsody
LeviCell
10x Genomics (Chromium)
NanoDrop ND-8000
Agilent 2100 Bioanalyzer
BioRad CFX96 Real-Time PCR System
SPT Labtech Mosquito
Agilent 4150 TapeStation
QIAGEN QIAcube
Agilent Bravo Automated Liquid Handling Platform
10x Genomics Visium CytAssist
ThermoFisher GeneTitan Multi-Channel (MC) Instrument
Gene Expression & Genotyping
Contact & Personnel
Services
Instruments
Sample Requirements
Getting Started
Core Resources
Acknowledge Core
Proper Citation:
University of Florida ICBR Gene Expression and Genotyping Core Core Facility, RRID:SCR_019145
What’s an RRID?
Contact
ICBR-Genotyping@ad.ufl.edu
ICBR-GeneExpression@ad.ufl.edu
(352) 273-8043
(352) 273-8036
Location
Cancer & Genetics Research Complex
178 CGRC
2033 Mowry Road
Gainesville FL 32610