NanoFCM – UF ICBR
NanoFCM
UF | ICBR Cytometry
NanoFCM: Nanoparticle Flow Cytometer analyzer
The
NanoFCM
is a nano-flow cytometry instrument optimized for measuring biological nanoparticles such as liposomes, extracellular vesicles (including OMVs and exosomes), and viruses. Equipped with two lasers, two fluorescence detectors, and one side scatter detector, the
NanoFCM
can measure particle size, size distribution concentration, and the presence of markers stemming from fluorescence labelling in a one-minute run time with as little as 30uL of sample. This device is compatible with a wide range of labelling strategies, such as GFP expression or immuno-labelling with fluorescently tagged antibodies (AlexaFluor and PE being the most used markers), nucleic acid dyes (SYTO products for instance), and membrane dyes (PkH67, Membright). The
NanoFCM
measures extracellular vesicles ranging from 40nm to 1μm, which covers most exosome and microvesicle fractions.
Summary of the NanoFCM features:
• Scatter sensitivity: <40 nm silica nanoparticles
• Particle concentration measurement (particles/mL)
• Single photon counting avalanche photodiode detectors
• Particle integration time control for high-sensitivity detection
• Real-time, individual particle trace event: removes risk of swarm-detection
• 30μL minimum sampling volume
• Simultaneous measurement of size and concentration for fluorescently labelled samples
• Calibration standards for size, concentration and fluorescence
• One-minute measurement time
• Scatter-triggering of signal detection: no risk of false-positive events compared to fluorescence triggering detection
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Proper Citation:
University of Florida ICBR Cytometry Core Facility, RRID:SCR_019119
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Contact
ICBR-Cytometry@ad.ufl.edu
(352) 273-6032
Locations
Cancer & Genetics Research Complex
Room 292 CGRC
2033 Mowry Road
Gainesville FL 32610
McKnight Brain Institute
Room L3-160 MBI
1149 Newell Drive
Gainesville FL 32610