(PDF) Knowledge-Based Dietary Intake Recommendations of Nutrients for Pediatric Patients with Maple Syrup Urine Disease

Article Knowledge-Based Dietary Intake Recommendations of Nutrients for Pediatric Patients with Maple Syrup Urine Disease Mayda Alrige 1,*, Haneen Banjar 2,3, Taghreed Shuaib 4, Amal Ahmed 1 and Raghad Gharbawi 1 1 Information Systems Department, Faculty of Computing and Information Technology, King Abdulaziz University, Jeddah 21577, Saudi Arabia 2 Computer Science Department, Faculty of Computing and Information Technology, King Abdulaziz University, Jeddah 21577, Saudi Arabia 3 Center of Artificial Intelligence in Precision Medicine, King Abdulaziz University, Jeddah 21577, Saudi Arabia 4 Department of Genitics Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah 21577, Saudi Arabia * Correspondence:

Abstract: Maple syrup urine disease (MSUD) is a metabolic disorder characterized by a difficulty to digest and process proteins necessary for growth. To monitor and maintain the ideal growth of chil- dren with MSUD, caregivers need to carefully control the consumption of harmful branched-chain amino acids (BCAAs). The dietary limits of amino acids for MSUD patients are recommended and controlled by pediatricians and metabolic dietitians according to age, height, weight, and the pre- vailing percentage of amino acids in the body. This study introduces an intelligent dietary tool called MSUD Baby Buddy for caregivers of MSUD patients that tracks the amino acids intake out of baby formulas for babies 0–6 months old. This tool aims to provide accurate recommendations of the appropriate daily intake of protein and BCAAs based on the patients’ data, plasma BCAAs, and formula preferences. We use a knowledge-based system, including knowledge acquisition and ver- ification, as well as knowledge management tool validation, and the ripple-down rules are em- Citation: Alrige, M.; Banjar, H.; ployed for building the system. MSUD Baby Buddy can support the maintenance of adequate amino Shuaib, T.; Ahmed, A.; Gharbawi, R. Knowledge-Based Dietary Intake acid levels and increase awareness about the control of BCAAs. The average usability of MSUD Recommendations of Nutrients for Baby Buddy is 84.25, indicating that the tool is intuitive and may help caregivers to easily determine Pediatric Patients with Maple Syrup the recommended doses of formula based on patients’ biometric data and preferred formula. On Urine Disease. Healthcare 2023, 11, the other hand, interviews with metabolic dietitians revealed some drawbacks, which were ad- 301. https://doi.org/10.3390/ dressed to further improve the tool. MSUD Baby Buddy is expected to help caregivers of MSUD healthcare11030301 patients to independently track nutrient intake and reduce the number of visits to the pediatrician Academic Editor: Abdel-Latif and metabolic dietitian. Mohamed Keywords: artificial intelligence; knowledge base; maple syrup urine disease; recommended die- Received: 18 September 2022 tary intake; pediatric patients Revised: 20 December 2022 Accepted: 10 January 2023 Published: 18 January 2023 1. Introduction Maple syrup urine disease (MSUD) is a rare inborn error of metabolism (IEM), with Copyright: © 2023 by the author. Li- censee MDPI, Basel, Switzerland. an estimated 1 in 185,000 newborns being diagnosed with this disease worldwide [1]. A This article is an open access article patient with MSUD cannot correctly break down proteins that are essential for growth, distributed under the terms and con- development, and tissue repair. For a body to gain nutrition from consumed food, they ditions of the Creative Commons At- must be broken down into the constituent amino acids. However, MSUD patients cannot tribution (CC BY) license (https://cre- break down the three branched-chain amino acids (BCAAs), namely, valine, leucine, and ativecommons.org/licenses/by/4.0/). isoleucine, from consumed food. This inability causes harmful substances to accumulate Healthcare 2023, 11, 301. https://doi.org/10.3390/healthcare11030301 www.mdpi.com/journal/healthcare Healthcare 2023, 11, 301 2 of 12 in the blood and urine, leading to metabolic crises and learning difficulties. If MSUD is left untreated, patients will likely deteriorate, slip into a coma, and possibly die. Therefore, caregivers and specialists must care for and monitor the health of MSUD patients, espe- cially aiming to control and reduce dangerous levels of BCAAs within the patients’ bodies [2]. MSUD patients must follow a strict low-protein diet to control and reduce the intake of BCAAs while maintaining adequate levels of protein, fluid, and energy for proper growth and development. Tracking the daily intake of BCAAs is challenging because their contents are not readily available on food labels or nutrition tables. Parents and guardians normally consult pediatricians or metabolic dietitians to obtain recommendations for nu- trient content quantities [3]. The BCAA levels that can be tolerated by children depend on age, height, weight, and BCAA concentration in blood plasma. As babies grow rapidly in their first 6 months, the recommended quantities must be reassessed every 2–4 weeks, and dietary nutrient content must be adjusted accordingly. Furthermore, parents or caregivers are always at risk of under-/overestimating the amino acid content of formula, threatening metabolic control [4]. To mitigate this issue, diet tracking has been studied for many diet- related chronic diseases [5], not only inherited metabolic chronic diseases [6]. Mobile health and dietary management systems are available for diet tracking to guide individu- als based on certain dietary recommendations and nutrition protocols [5]. For instance, the Metabolic Diet App is an online web application for patients with IEM, covering 15 types of IEMs. The personalized dashboard and management plan for tracking and plan- ning meals include an input for homemade recipes, a food diary for managing and track- ing daily intake, and a tracker of BCAA intake [6]. A similar mobile application (app) is Diet Assistant, which is a leucine calculator for MSUD patients, in which users can track their daily intake of leucine by registering every meal they eat on the app registry. These inputs are exported to a daily log for users to check. In addition, the app has a progress bar that indicates the amount of leucine consumed within the daily limit [7]. All these apps are great attempts to help to track diet and recommend nutrient intake for different chronic metabolic disorders; however, they fall short of translating the certain nutrient quantities into practical, meal-based daily limits, as caregivers often struggle to transfer the daily limit into formula doses throughout the day. Many parents and guardians fail to evaluate the amino acid limits according to the patient’s age, weight, and plasma BCAAs, and consequently cannot apply these limits to the baby formula daily dosages. To address this problem, we introduce MSUD Baby Buddy, an intelligent dietary tool, with the main aim of providing accurate recommendations of the appropriate daily intake of protein and BCAAs in the right amount of formula daily dosages. These recom- mendations take into account patients’ data, plasma BCAAs, and formula preferences. The proposed knowledge-based system can be used for tracking diets, helping caregivers of MSUD patients 0–6 months old to personalize diets based on biometric data. MSUD Baby Buddy provides easy formula dosage recommendations for MSUD patients based on weight, age, and plasma BCAAs while considering medical and commercial formula preferences. The general recommended nutrient content is based on gold-standard nutri- tion support protocols used by pediatricians and metabolic dietitians [8]. In addition, MSUD Baby Buddy provides customized nutrition recommendations based on formula preferences. MSUD Baby Buddy is intended to achieve the following objectives: 1. Calculate the recommended daily intake of fluid, protein, energy, and BCAAs (i.e., leucine, isoleucine, and valine) based on the patient’s BCAA levels, age, and weight; 2. With a rule-based system, recommend doses of medical and commercial formulas depending on BCAAs, fluid, protein, and energy using the calculated daily intake limits; 3. Provide general health tips for caregivers to maintain the metabolic health of MSUD patients. Healthcare 2023, 11, 301 3 of 12 2. Materials and Methods Software-based research has been conducted to develop and evaluate the knowledge-based MSUD Baby Buddy app, which was developed as a handy app that can be easily installed on the mobile phones of caregivers. This app assists in developing a knowledge base of the MSUD patient and includes steps of knowledge acquisition, veri- fication, and knowledge management tool validation. The ripple-down rules (RDRs) are used for building the system to support the diet of MSUD patients [9]. The app considers five variables: age, height, weight, plasma BCAAs, and type of formula (both commercial and medical). The variables and desired quantity of daily protein are used to recommend adequate BCAA intake to caregivers. For example, when the caregiver provides the pa- tient’s preferred formula and data, MSUD Baby Buddy presents the recommended daily food intake (formula dosage). 2.1. The Knowledge-Based System The flowchart to develop and evaluate the knowledge base of MSUD Baby Buddy is shown in Figure 1. The first phase concerns knowledge acquisition, where the knowledge base has been built using the RDRs, which is elaborated on in Section 3.2. The second phase is for knowledge management and validation, where, first, the nutrition manage- ment tool has been prototyped, elaborated on in Section 3.3; and, second, the full version of the tool (MSUD Baby Buddy) has been developed, elaborated on in Section 3. An open- ended questionnaire was used to test and evaluate the tool by parents, guardians, care- givers, and nursing or health care professionals of MSUD patients. Ethics approval was obtained from the Unit of Biomedical Ethics of the King Abdulaziz University Hospital (No. 229-21). Figure 1. Flowchart to develop and evaluate knowledge base of MSUD Baby Buddy. 2.2. Knowledge Acquisition Diet tracking has been widely studied, and diverse knowledge-based systems are available in medicine to recommend solutions for individuals based on certain protocols. The proposed MSUD Baby Buddy follows the RDRs to acquire and build the knowledge base [10]. Knowledge acquisition was aimed to create and refine rules, as depicted in Fig- ure 2. An RDR allows a domain expert to define the processing of incoming information for decision making. In addition, RDRs incrementally subdivide the object space into smaller partitions, aiming to classify all the objects from the same class to the same parti- tion. This partitioning can be established by the domain expert, ensuring an adequate sub- division [9]. Healthcare 2023, 11, 301 4 of 12 Figure 2. Knowledge acquired using RDRs [10]. Healthcare 2023, 11, 301 5 of 12 2.3. Knowledge Verification Then, we verified the knowledge acquired in the first step. This process took approx- imately 5 months, from 20 August 2021 to 21 January 2022. The MSUD nutritional facts were used to identify initial allowable limits of BCAAs and protein intake based on the patient’s age and weight [9]. A metabolic dietitian assisted in acquiring the details of the variables to provide a recommendation. The age variable was categorized into six differ- ent groups to meet the BCAA requirements, being classified on a monthly basis from 0 (birth) to 6 (half a year) months. Finally, we conducted a focus group discussion, with the participation of three metabolic dietitians and nutritionists/specialists, about formulating rules regarding types of formulas and units before recommending the type and quantity of formula for every patient. 2.4. Knowledge Management and Validation After verifying the knowledge base, we implemented user interfaces for the MSUD Baby Buddy app. We aimed to validate and verify the usability and adaptability of the created interfaces. Usability testing allows measurement of the ease of handling and clar- ity in understanding an app. As these attributes vary according to the user and personal knowledge, the attitude of the participants was noted to understand the easiness, simplic- ity, and friendliness of the interfaces and app [10]. We adopted the system usability scale (SUS) for testing usability [11]. The SUS is re- liable in the practical application and evaluation of various kinds of application systems. As the MSUD Baby Buddy app is intended for use worldwide, it must meet usability pa- rameters in line with MSUD nutrition protocols. The same standard and ease of use is required for adaptability and performance of required tasks by caregivers. After polishing and confirming the interfaces and reaching consensus about MSUD Baby Buddy usability, we started working on the back end of the app. Finally, we de- ployed unit testing according to relevant ethical procedures to evaluate the main func- tionalities of MSUD Baby Buddy [12]. 3. Results This section presents the results of the knowledge acquired using RDR. The second subsection presents the results of verifying with domain experts the dietary recommen- dations and nutrition protocols. The third subsection presents the results of tool validation through front-end and back-end testing. 3.1. Acquired Knowledge By obtaining the partitioning scheme and rules from the domain experts, we repre- sent the knowledge base using production rules. Other techniques for knowledge base representation include logical representation, semantic network representation, and frame representation. We chose production rules and in particular ripple-down rules to represent the knowledge base. Following this technique, we built an RDR tree, as shown in Figure 2, where each node contains a logical condition, explanation, and conclusion. A node can have at most two children. 3.2. Verification of Knowledge Base Two main categories of knowledge were verified. First, the daily allowable limits of the three BCAAs, protein, energy, and fluid were evaluated according to age (0–6 months), as listed in Table 1. Healthcare 2023, 11, 301 6 of 12 Table 1. Daily recommended intake of BCAAs, protein, and fluid for MSUD patients. Age Isoleucine Leucine Valine (mg) Protein (gm) Energy (kcal) Fluid (mL) (months) (mg) (mg) 1 40 66.5 46.5 3.45 120 100 2 48 79.5 55.5 3.34 120 100 3 56 92.5 64.5 3.16 120 100 4 48.5 56 56 3 120 100 5 40.5 68 47 2.84 120 100 6 47.5 80 55 2.68 120 100 Second, the nutrient content of six types of formulas was collected, as listed in Table 2. This table shows commercial (first three rows) and medical (last three rows) formulas suitable for children with IEMs. The medical formulas do not contain BCAAs, indicating their suitability for MSUD patients. However, medical formulas are generally more ex- pensive than commercial or dietary ones owing to their sustainability and production un- der supervision of dietitians, who know the correct contents for required metabolic rates and specifications, being more useful when considering the patient’s body, characteristics, and BCAA requirements. Table 2. Nutrient content of six common formulas. Isoleucine Formula Leucine (mg) Valine (mg) Protein (gm) Energy (kcal) (mg) Nan 1 ® 106.4 51.6 53.6 12.3 506 Similac ® 1079 573 641 10.83 526 Aptamil ® 1020 480 520 4.7 485 Ketonex-1 ® 0 0 0 15 480 Anamix ® 0 0 0 13.1 466 Comida ® 0 0 0 12 205 Regarding the recommended intake of BCAAs, protein, energy, and fluid according to age (0–6 months) shown in Table 2, two metabolic dietitians expressed the need for a standard unit, as it could help MSUD patients’ caregivers to easily control the intake of the formulas listed in Table 2. The results of standardization are reported as equations in Appendix A. This appendix explains the dietary formulations where the dosage amount is calculated according to the selected commercial formula’s and infant’s weight (w). Ad- ditionally, the rules for diet plans are detailed in Appendix B. This appendix outlines the feeding rules based on the patient’s daily feeding plan after standardizing the formula scoop size. The doses per type of formula are specified in units of 5 g, which is equivalent to one spoonful (provided by the metabolic dietitian). This dose unit is used to facilitate complex calculations of the BCAAs and dosage. The resultant outlined tables recommend that, based on a 5 g scoop size, the nutrient content outlined in Table 2 and the rules spec- ified in Appendices A and B can be considered to build the knowledge base of MSUD Baby Buddy. 3.3. Validation of Knowledge-Based Tool We validated the tool usability using SUS for the front-end part and unit and inte- gration testing for the back-end part of the tool. For the front-end part, we tested the in- terfaces on 10 MSUD caregivers (n = 10). These caregivers completed the SUS survey and provided feedback through open-ended questions. All respondents were parents (eight women and two men) of MSUD patients. Based on the answers to the usability question- naire, the average SUS score was 84.25, indicating that MSUD Baby Buddy is usable and Healthcare 2023, 11, 301 7 of 12 may help caregivers of MSUD patients to determine the appropriate doses of formula based on four variables: age, weight, plasma BCAAs, and formula preference. The validation of the back-end part of the tool was carried out using unit testing for the three main functions of the knowledge-based system. The first function enables MSUD patients’ caregivers to provide required data and fill out questionnaires and forms related to the input variables, as shown in Figure 3. The second function allows users to select their preferred formula from medical and commercial options (Figure 4). The validity is checked through studying and researching the formula composition for suitability with the patient’s body and reaction on consumption according to the recommended daily in- take. Finally, based on calculations, the recommended daily intake of energy, protein, and allowable limits of BCAAs (i.e., valine, leucine, and isoleucine) is shown on the interface (Figure 5). The information about unit testing for the three functions is listed in Table 3. Figure 3. MSUD patient profile. . Figure 4. Allowing the caregivers to choose their preferred medical/commercial formulas. Figure 5. Recommending the appropriate daily doses of formula. Healthcare 2023, 11, 301 8 of 12 Table 3. Unit testing for three functions of MSUD Baby Buddy. Unit Test Description Input Expected Result Actual Result Pass/Fail ID After caregiver submits Verify input plasma Leucine: 80 plasma BCAA, the system Display recommended 1 BCAA by inserting Isoleucine: 17 Pass shows recommended daily daily intake for patient valid data Valine: 55 intake Verify input plasma Leucine: 80 Display error message in Display error message 2 BCAA by inserting in- Isoleucine: 17 Pass invalid field in all fields valid data Valine: – Verify correct recom- Display necessary limits Display necessary limits mended daily intake for BCAAs, protein, en- 3 No input data for BCAAs, protein, en- Pass based on weight and ergy, and fluid for pa- ergy, and fluid for patient age of patient tient Similac® (commer- Select commercial for- Caregivers select preferred Show recommended 4 cial formula) se- Pass mula formula daily intake of formula lected from image Ketonex-1 (medical Caregivers select preferred Show recommended 5 Select medical formula formula) selected Pass formula daily intake of formula from image Select commercial for- Display error message Display error message 6 No selection Pass mula “Please select formula “ “Please select formula” Display error message Display error message 7 Select medical formula No selection Pass “Please select formula” “Please select formula” These results, together, suggest that MSUD Baby Buddy is technically valid and can deliver the three main functionalities outlined in Figure 3, Figure 4, and Figure 5, respec- tively. Brief interviews with caregivers and their responses to the open-ended questions revealed that continuous monitoring of the metabolic rate is considered essential for pre- venting severe side effects from overtaking a dangerous BCAA. In addition, caregivers considered that pediatricians are very busy, increasing the waiting time of patients at the hospital, whereas quality time is reduced for families with less access to specialists when- ever they require appointments in between. Moreover, caregivers considered the waiting times as tiring, and that patients need to manage the amino acid calculations at home because there are no adequate facilities available at hospitals with comfortable environ- ments for the pediatric patients. Using assistive tools such as the proposed MSUD Baby Buddy may increase confi- dence and reduce anxiety and fear of caregivers in managing the disease. In addition, practitioners may schedule appointments and follow-ups in longer intervals. Hence, de- spite limited access to specialists, continuous monitoring may be enabled by using tools such as MSUD Baby Buddy. Moreover, metabolic and diet control for BCAAs may be eas- ily achieved using a diet-tracking app if patients or parents are educated about the formu- las. The role of dietitians has been concerned with follow-ups, and interviews with parents in this study showed that the importance of a metabolic dietitian has been overempha- sized by health agents. Nevertheless, regular appointments with a dietitian for monitoring the blood levels of amino acids in children are common. In fact, close monitoring and regular appointments with practitioners are necessary for children to suitably control MSUD. We also found service and compliance problems that should be investigated by families regarding nutritional monitoring. Healthcare 2023, 11, 301 9 of 12 4. Discussion MSUD is an IEM that exposes infant patients to a great risk of suffering from hyper- catabolism [3,4]. Moreover, MSUD can cause metabolic crises and learning difficulties. If left untreated, MSUD patients may deteriorate, slip into coma, and die, in the worst case. Managing the diet of an MSUD patient primarily affects caregivers, who are responsible for maintaining a low-protein diet. After a newborn is discharged from hospital, caregiv- ers suffer from a psychological burden caused by the MSUD diagnosis and responsibility for the dietary therapy [4]. Dietitians who treat MSUD should support the families psy- chologically, considering the four main aspects of IEMs as rare, metabolic, chronic, and genetic diseases [4]. The child’s health condition depends on the care provided by the family at home, and self-tracking mobile health solutions can greatly help in treatment [13]. From a technical perspective, self-tracking involves digital systems and devices that allow users to collect, analyze, and reflect on data [14,15]. In this sense, self-tracking may help caregivers to pay special attention to the daily needs of MSUD patients. MSUD Baby Buddy allows users to regularly update the patient’s weight, age, and plasma BCAA lev- els to properly adjust the recommended daily intake of protein, BCAAs, and formula doses. The customization of dietary guidance has been widely studied to help to manage diet-related chronic diseases or maintain a healthy lifestyle [16]. In particular, personal- ized nutrition provides dietary recommendations according to specific biological require- ments based on a person’s health status and goals [17]. Personalized nutrition is deline- ated by three elements: (1) science and data, (2) professional education and training, and (3) guidance and therapeutics. Similarly, we have developed a technological solution that further increases personalized nutrition guidance, products, and services by focusing on MSUD patients. MSUD is an IEM for which protein and BCAA intake must be carefully controlled. Solutions based on artificial intelligence and decision making will likely help caregivers to closely monitor the patient’s protein intake based on medical and commer- cial formulas. Additional research is required to expand the knowledge base and include other types of commercial and medical formulas. The six types of formulas included in this re- search were selected based on a preliminary exploratory questionnaire intended to iden- tify the common types used in a city. However, these formulas are not standard. For the knowledge base to be applicable to a larger population of MSUD patients, we must con- sider other standard types of formulas. In addition, breast milk must be included in the knowledge base. Some mothers indicated that they prefer to breastfeed their children but are concerned with the BCAA contents, which vary from one mother to another. Although an average content can be obtained, careful monitoring by metabolic dietitians and clinical nutritionists will be required for safe breastfeeding. 5. Conclusions The MSUD Baby Buddy app aims to provide caregivers accurate recommended daily intake of proteins, BCAAs, and fluids for infants 0–6 months of age through calculations based on the MSUD patient’s age, weight, and plasma BCAA levels. The knowledge- based system allows caregivers to feed patients with their preferred medical or commer- cial formulas and provide adequate intake of protein, energy, and fluids. The average sys- tem usability score is 84.25, indicating that MSUD Baby Buddy is technically usable and would help caregivers to easily determine the recommended formula dosage based on the MSUD patient’s biometric data and preferred formulas. This tool seems promising to sup- port caregivers of MSUD patients in independently tracking protein intake and reducing the required number of visits to pediatricians and metabolic dietitians. Author Contributions: Conceptualization: M.A. and H.B.; methodology: M.A.; software: A.A. and R.G.; validation: T.S., A.A. and R.G.; formal analysis: A.A.; data curation: A.A. and R.G.; writing— original draft preparation: M.A.; writing—review and editing: H.B.; visualization: A.A. and R.G.; Healthcare 2023, 11, 301 10 of 12 funding acquisition: M.A. All authors have read and agreed to the published version of the manu- script. Funding: This research was funded by the Deanship of Scientific Research (DSR) at King Abdelaziz University, Jeddah, Saudi Arabia, under grant no. IFPHI 376-612-2020. The authors are grateful to the DSR for the technical and financial support. Institutional Review Board Statement: This study was conducted in accordance with the approval of the Unit of Biomedical Ethics (research committee) of the Medical School at King Abdelaziz Uni- versity. Informed Consent Statement: Informed consent was obtained from all subjects involved in the study. Data Availability Statement: The following link is provided to the project repository. It is public for everyone to install the software and start using it: https://github.com/mal- raegi/MSUD_Baby_Buddy.git, accessed on 2 September 2022. Acknowledgments: We thank all the registered dietitians who assisted us throughout the building of the knowledge base, especially Wafa Alabyad for her continued support. Conflicts of Interest: Authors declare no conflict of interest. Abbreviations MSUD Maple syrup urine disease IEM Inborn error of metabolism RDRs Ripple-down rules SUS System usability score Appendix A This appendix explains the dietary formulations where the dosage amount is calcu- lated according to the selected commercial formula’s and infant’s weight (w). Similac 100 × (val with w) VAL_sim_res = 641 100 × (leu with w) LEU_ sim_res = 1079 10.83 × VAL_sim_res Protein_ sim _res = 100 Nan1 100 × (val with w) VAL_Nan_res = 53.6 100 × (leu with w) LEU_ Nan _res = 106.4 12.3 × VAL_Nan_res Protein_ Apt _res = 100 Aptamil 100 × (val with w) VAL_Apt_res = 520 100 × (leu with w) LEU_ Apt _res = 1020 4.7 × VAL_Apt_res Protein_ Apt _res = 100 Dosage calculation according to selected medical formula: Healthcare 2023, 11, 301 11 of 12 Ketonix-1 Pro_keto = protein content of user-selected commercial formula − plasma protein content and weight of patient 100 × Pro_keto Protein_keto_res = 15 480 × Protein_keto_res Energy_ keto_res = 100 Anamix Pro_Ana = protein content of user-selected commercial formula − plasma protein content and weight of patient 100 × Ana_keto Protein_Ana_res = 13.1 466 × Protein_Ana_res Energy_ Ana_res = 100 Comida Pro_Com = protein content of user-selected commercial formula − plasma protein content and weight of patient 100 × Com_keto Protein_ Com _res= 12 205 × protein_Com _res energy_ Com _res = 100 Appendix B This appendix outlines the feeding rules based on the patient’s daily feeding plan after standardizing the formula scoop size. Fluid_b= 100 × w Feeding = Fluid_b/5 If (Feeding == 25) { ¾ scoops (5 g) 25 mL of warm water 8 times a day every 3 h} If (Feeding == 30) { 1 scoop (5 g) 30 mL of warm water 8 times a day every 3 h} If (Feeding == 40) { 1 ¾ scoops (5 g) 40 mL of warm water 8 times a day every 3 h} If (Feeding == 60) { 2 scoops (5 g) 60 mL of warm water 8 times a day every 3 h} If (Feeding == 100) { 3 scoops (5 g) 100 mL of warm water 8 times a day every 3 h} If (Feeding == 120) { 4 scoops (5 g) 120 mL of warm water 8 times a day every 3 h} Healthcare 2023, 11, 301 12 of 12 If (Feeding == 160) { 5 scoops (5 g) 160 mL of warm water 8 times a day every 3 h} If (Feeding == 180) { 6 scoops (5 g) 180 mL of warm water 8 times a day every 3 h} References 1. Bharadwaj, A.; Wahi, N.; Saxena, A. Occurrence of Inborn Errors of Metabolism in Newborns, Diagnosis and Prophylaxis. Endocr. Metab. Immune Disord. Drug Targets 2021, 21, 592–616. https://doi.org/10.2174/1871530321666201223110918. 2. 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