Publications | The Wei Lab

Publications | The Wei Lab
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Publications
CMS
Pre-Prints
3. Optical Metabolic Imaging of the Tricarboxylic Acid Cycle
R.Chadha
, P. Kocheril
, A. Colazo
, E. Kapelczak, B. Suen, Z. Yang, T. TeSlaa, and
L. Wei*.
bioRxiv
2026
‡ denotes equal contribution
2. Deep Learning-Augmented Stimulated Raman Imaging for Cell-Type-Specific Metabolic Profiling in Live Neuronal Co-Cultures
L-E. Lin, X. Bi, A. Colazo, H. Wang, and
L. Wei*
ioRxiv
2025
1. Endothelial AGO1 Drives Vascular Inflammation and Atherosclerosis via a Non-Canonical Nuclear Mechanism
X. Liu*, D.Yuan, Y. Luo, X. Tang, A. Tapia, N. Malhi, R. Chadha, S. Tiwari, P. Swiderski, K-C. Wang, M. Kortylewski, N. Pardi,
L. Wei
, W. Huang, Z. Chen*.
ioRxiv.
2025
51. Towards accurate predictions of bond-selective fluorescence spectra
P. Kocheril, R. Leighton, N. Naji, D. Lee, H. Wang, J. Du, and
L. Wei
*.
Journal of Chemical Physics
2026
, 164, 130901.
50. Far-field single-molecule vibrational spectroscopy and imaging
P. Kocheril, H. Wang, R. Leighton, D. Lee, N. Naji, W. Min, and
L. Wei
*.
Chemical Science
2026,
17, 5297-5316.
49. Mapping Endothelial-Macrophage Interactions in Diabetic Vasculature: Role of TREM2 in Vascular Inflammation and Ischemic Response
N.K. Malhi, Y. Luo, M. Chen, X. Tang, D. Yuan, R. Chadha, A. Tapia, S. Yin, X. Liu, M. Qi, M. Reddy, J. Sun, J. Otto,
L. Wei
, J. P. Cooke, E. Lee, R. Natarajan, K.W. Southerland, Z.B. Chen*.
bioRxiv.
2025
48. GFP-Free Live-Neuron Quantitative Imaging Reveals Compartmentalization and Growth Dynamics of PolyQ Aggregates
X. Bi, B. Suen, L-E. Lin, K. Miao,
L. Wei*.
Proceedings of the National Academy of Sciences
2026
, 123, 4, e2505321123.
47. Single-Cell Metabolic Imaging Reveals Glycogen-Driven Adaptations in Endothelial Cells
R. Chadha, B. Yang, D. Yuan, E. Kapelczak, P. Kocheril, A. Tapia, S. Mahajan, A.Colazo, N. Malhi, J. Ambarian, X. Liu, T. TeSlaa, Z. Chen*,
L. Wei*
Advanced Science
2025
, 13, 7, e08517
(*Co-corresponding)
46. Single-molecule Vibrational Thermometry
P. Kocheril, D. Lee, N. Naji, R. Chadha, R. Leighton, H. Wang, and
L. Wei*.
Journal of Physical Chemistry B.
2025
129
, 34, 8788–8797.
45. Two-dimensional bond-selective fluorescence spectroscopy: Violations of the resonance condition, vibrational cooling rate dispersion, and super-multiplex imaging
P. Kocheril, J. Du, H. Wang, R. Leighton, D. Lee, Z. Yang, N. Naji, A. Colazo, and
L. Wei*.
Chemical Science
2025
16
, 14905-14918.
This article is part of the themed collections:
2025 Chemical Science Covers
and
Most popular 2025 sensing & imaging articles
44. Visible Enhanced Stimulated Raman Scattering Microscopy via Frequency-Doubling of Picosecond Pulses
A. Colazo, K. Miao, L-E. Lin, R. Chadha, C. Qian, X. Bi, P. Kocheril,
L. Wei*
Biomedical Optics Express
2025
16
, 2430-2439
43. Illuminating Life Processes by Vibrational Probes
N. Qian, Z. Zhao, E. Khoury, X. Gao, C. Canela, Y. Shen, L. Shi, L. Shi, F. Hu,
L. Wei
, Wei Min*.
Nature Methods
2025
22
, 928–944.
42. Merging Vibrational Spectroscopy with Fluorescence Microscopy: Combining the Best of Two Worlds
N. Qian*, H. Xiong*,
L. Wei
, L. Shi*, and Wei Min*.
Annual Review of Physical Chemistry
2025
76
, 279-301.
(*Co-corresponding)
41. High-Throughput Volumetric Mapping Facilitated by Active Tissue SHRINK
L. Lin, A. Colazo, X. Bi, J. Du,
L. Wei
.*
Small Methods
2025
10
, 2,
2500382.
40. Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video rate
D. Lee, H. Wang, P. Kocheril, X. Bi, N. Naji,
L. Wei*
Optica
2025
, 12 (2), 148–157.
39. Room-Temperature Single-Molecule Infrared Imaging and Spectroscopy through Bond-Selective Fluorescence
H. Wang, P. Kocheril, Z. Yang, D. Lee, N. Naji, J. Du, L. Lin,
L. Wei
.*
Angewandte Chemie
2024
, 63 (52), e202413647.
38. Nitrile Vibrational Lifetimes as Probes of Local Electric Fields
P. Kocheril,
H. Wang
D. Lee
N. Naji, and
L. Wei
.*
Journal of Physical Chemistry Letters
2024
, 15, 19, 5306–5314.
**Supplementary Cover
37. Quantitative Real-Time Analysis of Living Materials by Stimulated Raman Scattering Microscopy
C. Qian†, H. Liu†, P. Chittur, R. Chadha, Y. Yao, J. Kornfield, D. Tirrell*, and
L. Wei
.*
Analytical Chemistry.
2024,
96, 17, 6540–6549. (†Equal contributions, *Co-corresponding)
**Supplementary Cover
36. Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level
R. Chadha†, J.Guerrero†,
L. Wei
*, L. Sanchez.*
ACS Central Science.
2024
, 10 (4), 758-774. (†Equal Contributions; *Co-corresponding)
**
Supplementary Cover
35. Bond-selective Fluorescence Imaging with Single-molecule Sensitivity
H. Wang†, D. Lee†, Y. Cao, X. Bi, J. Du, K. Miao &
L. Wei
*,
Nature Photonics
2023
, 17, 846–855. (†Equal Contributions)
"BonFIRE Ignites a New Era in Microscopy: Unveiling the Molecular Diversity of Life in Stunning Detail
, featured by SciTechDaily.
"Microscopy Method Uses Infrared Light to Image Molecules with Vibrational Contrast
, featured by Photonics Spectra.
"​
New Combination of Imaging Techniques Could Revolutionize Microscopy
, featured by Technology Networks.
34. Computational Design of Molecular Probes for Electronic Preresonance Raman Scattering Microscopy
J. Du†, X. Tao†, T. Begušić*, &
L. Wei
*,
Journal of Physical Chemistry B.
2023
127 (22), 4979-4988.
(†Equal Contributions, *Co-corresponding)
33. Toward the Next Frontiers of Vibrational Imaging
H. Wang, D. Lee, &
L. Wei
*,
Chemical & Biomedical Imaging
2023,
1 (1), 3-17.
32. Bringing Vibrational Imaging to Chemical Biology With Molecular Probes
J. Du, H. Wang & L. Wei*,
ACS Chemical Biology
2022,
17 (7), 1621-1637.
**Supplementary Cov
er
31. Label-free Super-resolution Imaging Enabled by Vibrational Imaging of Swelled Tissue and Analysis
K. Miao, L. Lin, C. Qian &
L. Wei
*,
Journal of Visualized Experiments.
2022,
183, e63824.
30. Alkyne-Tagged Raman Probes for Local Environmental Sensing by Hydrogen–Deuterium Exchange
X. Bi†, K. Miao† &
L. Wei
*,
Journal of the American Chemical Society.
2022
. 144 (19), 8504-8514.
†Equal Contributions
29. Multicolor Photoactivatable Raman Probes for Subcellular Imaging and Tracking by Cyclopropenone Caging
J. Du &
L. Wei
*,
Journal of the
American
Chemical Society.
2022
. 144 (2), 777-786.
**Supplementary Cover
28. Super-Resolution Label-free Volumetric Vibrational Imaging
C. Qian†, K. Miao†, L. Lin, X. Chen, J. Du,
L. Wei
*,
Nature Communications.
2021
3648. †Equal Contributions
Microscopy method achieves superresolution without labels
”,
featured by C&EN.
​"
C&EN’s Year in Chemistry 2021
27. High spatial-resolution imaging of label-free in vivo protein aggregates by VISTA
L. Lin, K. Miao, C. Qian,
L. Wei
*,
Analyst.
2021,
146, 4135-4145.
**Supplementary Cover
26. Toward Photoswitchable Pre-resonance Stimulated Raman Probes
D. Lee, C. Qian, H. Wang, L. Lei, K. Miao, J. Du, D. M.  Shcherbakova, V. V. Verkhusha, L.V. Wang, and
L. Wei
*,
Journal of Chemical Physics
2021
.154, 135102.
- As a part of the 2021 JCP Emerging Investigators Special Collection
- JCP Editors’ Choice 2021
- Featured article
25. Visualizing Subcellular Enrichment of Glycogen in Live Cancer Cells by Stimulated Raman Scattering
D. Lee, J. Du, R. Yu, Y. Su, J. Heath, and
L. Wei
*,
Analytical Chemistry.
2020
. 92, 13182.
24. Raman-guided Subcellular Pharmaco-Metabolomics for Metastatic Melanoma Cells
J. Du†, Y. Su†, C. Qian, D. Yuan, K. Miao, D. Lee, A. Ng, R. Wijker, A. Ribas, R. Levine, J. Heath*, and
L. Wei
*,
Nature Communications.
2020
. 11, 4830.
†Equal Contributions
"Cancer’s Hidden Vulnerabilities
” featured by ScienMag.
"Metabolic Processes in Cancer Cells Studied via Pharmaco-Metabolomics Approach
” featured by Genetic Engineering & Biotechnology News.
“Exposing Cancer's Vulnerabilities Using Specialized Microscopy”
featured by Technology Networks.
23. Live-Cell Imaging and Quantification of PolyQ Aggregates by Stimulated Raman Scattering of Selective Deuterium Labeling
K. Miao and
L. Wei
*,
ACS Central Science.
2020
, 4, 478.
22. Volumetric Chemical Imaging by Clearing-Enhanced Stimulated Raman Scattering Microscopy
M. Wei†, L. Shi†, Y. Shen, Z. Zhao, A. Guzman, L. J. Kaufman, L. Wei* and Wei Min*,
Proceedings of the National Academy of Sciences.
2019
. USA, 116, 6608. (†Equal Contributions *Co-corresponding)
21. Stimulated Raman excited fluorescence spectroscopy and imaging
H. Xiong†, L. Shi†, L. Wei, Y. Shen, R. Long, Z. Zhao and W. Min*,
Nature Photonics
2019
. 13, 412.
†Equal Contributions
Peer-Reviewed Publications
Book chapters:
1. “Chapter 20 - Stimulated Raman scattering imaging with small vibrational probes”
H. Wang, J. Du, D. Lee, and
L. Wei.
Stimulated Raman Scattering Microscopy, Techniques and Applications, 2022, Pages 289-310
Prior to Caltech:
20. Electronic Resonant Stimulated Raman Scattering Micro-Spectroscopy
H. Xiong†, L. Shi†,
L. Wei
, Y. Shen, R. Long, Z. Zhao and W. Min, Nat. Photonics, 13, 412 (2019). †Equal Contributions
19. Electronic pre-resonance stimulated Raman scattering microscopy
L. Wei
and W. Min, J. Phys. Chem. Lett. 9, 4294 (2018).
Cover
18. Operando, three dimensional, and simultaneous visualization of ion depletion and lithium growth by stimulated Raman scattering microscopy
Q. Cheng†,
L. Wei†
, Z. Liu, N. Ni, Z. Sang, B. Zhu, W. Xu, L.-Q.Chen, W. Min and Y. Yang, Nat. Commun., 9, 2942 (2018). †Equal contribution.
17. Supermultiplexed optical imaging and barcoding with engineered polyynes
F. Hu, C. Zeng, R. Long, Y. Miao,
L. Wei
, Q. Xu and W. Min, Nat. Methods, 15, 194(2018).
16. Super-multiplex vibrational imaging
L. Wei
, Z. Chen, L. Shi, R. Long, A. V. Anzalone, L. Zhang, F. Hu, R. Yuste, V. W. Cornish and W. Min, Nature, 544, 465 (2017).
A larger palette for biological imaging
” featured by Nature News & Views.
New dyes enable super-multicolor imaging
”, featured by Chem & Engn News.
Good vibrations for super-multiplexed imaging
” featured by Nature Methods.
New microscopy method breaks color barrier of optical imaging
” featured by Phys.org.
Expanding Bioimaging's Palette
” featured by Optics & Photonics News.​
15. Live-cell bioorthogonal chemical imaging: stimulated Raman scattering microscopy of vibrational probes
L. Wei
, F. Hu, Z. Chen, Y. Shen, L. Zhang and W. Min, Acc. Chem. Res.,49,1494 (2016).
14. Bioorthogonal chemical imaging of metabolic activities in live mammalian hippocampal tissues with stimulated Raman scattering
F. Hu, M. Lamprecht,
L. Wei
, B. Morrison and W. Min, Sci. Rep.,6, 39660 (2016).
13. Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling
L. Wei
, Y. Shen, F. Xu, F. Hu, J. K. Harrington, K. L. Targoff and W. Min. ACS Chem. Bio.10, 901 (2015).
Cover
12. Vibrational imaging of glucose uptake activity in live cells and tissues by stimulated Raman scattering
F. Hu, Z. Chen, L. Zhang, Y. Shen,
L. Wei
and W. Min. Angew. Chem. Int. Ed. 54, 9821 (2015).
11. Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman Scattering
L. Wei
, F. Hu, Y. Shen, Z. Chen, Y. Yu, C. -C. Lin, M. C. Wang and W. Min. Nat. Methods, 11, 410 (2014).
10. Multicolor live-cell chemical imaging by isotopically edited alkyne vibrational palette
Z. Chen, D. Paley,
L. Wei
, A. Weisman, R. Friesner, C. Nuckolls and W. Min. J. Am. Chem. Soc. 136, 8027 (2014).
9. Live-cell quantitative imaging of proteome degradation by stimulated Raman scattering
Y. Shen, F Xu,
L. Wei
, F. Hu and W. Min, Angew. Chem. Int. Ed.53, 5596 (2014).
8. Vibrational imaging of choline metabolites in live cells by stimulated Raman scattering coupled with isotope-based metabolic labeling
F. Hu,
L. Wei
, C. Zheng, Y. Shen and W. Min, Analyst, 139, 2312 (2014).
Cover
7. Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy
L. Wei
, Y. Yu, Y. Shen, M. Wang and W. Min, Proc. Natl. Acad. Sci. USA,110, 11226 (2013).
6. Frustrated FRET for high-contrast high-resolution two-photon imaging
F. Xu,
L. Wei
, Z. Chen and W. Min. Opt. Express, 12, 14097 (2013).
5. What can stimulated emission do for bio-imaging?
L. Wei
and W. Min. Ann. N. Y. Acad. Sci., 1293,1 (2013).
4. Pump-probe optical microscopy for imaging non-fluorescent chromophores
L. Wei
and W. Min. Anal. Bioanal. Chem.403, 2197 (2012).
3. Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag
E. Gatzogiannis, Z. Chen,
L. Wei
, R. Wombacher, Y. -T. Kao, G. Yefremov, V. C. Cornish and W. Min. Chem. Commun. 48, 8694 (2012).
2. Extending the fundamental imaging-depth limit of multi-photon microscopy by imaging with photo-activatable fluorophores
Z. Chen†,
L. Wei†
, X. Zhu† and W. Min. Opt. Express 20, 18525 (2012).  †Equal contribution
1. Stimulated emission reduced fluorescence microscopy: a concept for extending the fundamental depth limit of two-photon fluorescence imaging
L. Wei
, Z. Chen and W. Min. Biomed. Opt. Express 3, 1465 (2012).
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