The EUCOMM program | IMPC | International Mouse Phenotyping Consortium

The EUCOMM program | IMPC | International Mouse Phenotyping Consortium
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Human Diseases
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Latest IMPC Papers
Data Supporting IMPC Papers
Pain Sensitivity Associated Genes
Essential Genes - Linking to Disease
Essential Genes - Translating to Other Species
Sexual Dimorphism
Genes Critical for Hearing Identified
Genetic Basis for Metabolic Diseases
Papers Using IMPC Resources
News
Blog
Help
IMPC Cloud
About the IMPC
Institutional Members
Corporate Associates
Collaborations
Funding
Animal Welfare
About KOMP
Awards of Excellence
Training & Workshops
Workshops
Training
Getting Started with IMPC Data
IMPC Data Generation
How to Use Gene Pages
Citing IMPC Data
Allele design
IMPC Data Collections
Late Adult Data
Histopathology
Essential genes
Embryo Development
Cardiovascular
Accessing the Data
Latest Data Release
Access via API
Access via FTP
Batch query
Advanced Tools
Disease Models
PhenoDCC Tools
GenTaR
OpenStats
IMPReSS
Embryo Viewer
Software
Latest IMPC Papers
Data Supporting IMPC Papers
Pain Sensitivity Associated Genes
Essential Genes - Linking to Disease
Essential Genes - Translating to Other Species
Sexual Dimorphism
Genes Critical for Hearing Identified
Genetic Basis for Metabolic Diseases
Papers Using IMPC Resources
Genes
Phenotypes
Help, News, Blog
About IKMC
The EUCOMM program
About IKMC
The EUCOMM program
EUCOMM
The European Conditional Mouse Mutagenesis Program is founder member
and European cornerstone of the International Knockout Mouse Consortium
(IKMC).
EUCOMM
contributes the largest fraction of conditionally trapped
and targeted genes in mouse C57BL/6N embryonic stem (ES) cells to the
IKMC.
EUCOMM
vectors, mutant ES cells and mutant mice are distributed
worldwide, enabling functional genomics research in a standardized and
cost-effective manner by a much wider biomedical research community than
has been possible previously.
EUCOMM
mutant ES cells and vectors can be obtained from the European Mouse Mutant Cell Repository (EuMMCR).
EUCOMM
mutant mice are archived and distributed by the European Mouse Mutant Archive (EMMA).
EUCOMM Farewell Meeting Participants
EUCOMM Project Information
Main Objectives
Generation, archiving, and world-wide distribution of up to 12.000
conditional mutations across the mouse genome in mouse embryonic stem
(ES) cells
• Establishment of a limited number of mouse mutants from this resource
Mutagenesis Strategies
• Conditional gene trapping – random approach for expressed genes
• Conditional targeted trapping – directed approach, used for expressed genes
• Conditional gene targeting – directed approach, used for non-expressed genes
Networking
Generation of European mouse functional genomics platform by
synergistic integration of EMMA, EUMODIC, EURExpress, FLPFLEX ,FunGenES,
PRIME and others
• Cornerstone of world-wide mouse mutagenesis effort, member of the International Knockout Mouse Consortium (IKMC)
Societal Benefits
• Improvement of health research
• Speedup of drug development, therapy and diagnosis
• Competitive advantage for pharmaceutical industry
Funding & Structure
• Funded by European Union (FP 6, Priority 1 “Life Sciences, Genomics and Biotechnology for Health”)
• EU funding: up to 13 million Euros
• Integrated Project
• Consortium: 9 participants from four European countries
EUCOMM Scientific Advisory Board
Phil Avner, Paris, France
David Beier, Boston, MA, USA
Pierre Chambon, Strasbourg, France
Deanna Church, Bethesda, MD, USA
Frank Grosveld, Rotterdam, The Netherlands
Monica Justice, Houston, TX, USA
George Kollias, Vari, Greece
Björn Löwenadler, Mölndal, Sweden
Bernard Malissen, Marseille-Luminy, France
Andrew McMahon, Cambridge, MA, USA
H. Earl Ruley, Nashville, TN, USA
Phil Soriano, New York, NY, USA
EUCOMM Coordination
Wolfgang Wurst
Allan Bradley
EUCOMM Management
Cornelia Kaloff
Derek Matthews
Karin Simmerl
EUCOMM Participants
Helmholtz Zentrum München, German Research Center for Environmental Health GmbH (formerly GSF), Munich/Neuherberg, Germany
Wolfgang Wurst, Martin Hrabé de Angelis, Joel Schick, Andreas
Hörlein, Cornelia Kaloff, Claudia Seisenberger, Antje Bürger, Karin
Simmerl
The Wellcome Trust Sanger Institute Hinxton, Cambridge, UK
Allan Bradley, William C. Skarnes, Barry Rosen, Anthony West, Wendy Bushell, Vivek Iyer, Ramiro Ramirez-Solis, Derek Matthews
University of Frankfurt, Germany
Harald von Melchner, Frank Schnütgen
Charité, Berlin, Germany
Patricia Ruiz
University of Technology, Dresden, Germany
Francis Stewart, Konstantinos Anastassiadis, Andrea Kranz
Institut Clinique de la Souris, Strasbourg, France
Yann Hérault, Marie-Christine Birling, Guillaume Pavlovic, Mohammed Selloum
European Molecular Biology Laboratory (EMBL) Monterotondo, Italy
Nadia Rosenthal
Medical Research Council (MRC), Mammalian Genetics Unit, Harwell, UK
Steve Brown
Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Glauco Tocchini-Valentini, Brendan Doe
EUCOMM Publications
2012
Highly-efficient, fluorescent, locus directed cre and FlpO deleter mice on a pure C57BL/6N genetic background.
Birling MC, Dierich A, Jacquot S, Hérault Y, Pavlovic G.
Genesis. 2012 Jun;50(6):482-9. PMID:
22121025
DOI:
10.1002/dvg.20826
The mammalian gene function resource: the international knockout mouse consortium.
Bradley A, Anastassiadis K, Ayadi A, Battey JF, Bell C, Birling MC,
Bottomley J, Brown SD, Bürger A, Bult CJ, Bushell W, Collins FS,
Desaintes C, Doe B, Economides A, Eppig JT, Finnell RH, Fletcher C, Fray
M, Frendewey D, Friedel RH, Grosveld FG, Hansen J, Hérault Y, Hicks G,
Hörlein A, Houghton R, Hrabé de Angelis M, Huylebroeck D, Iyer V, de
Jong PJ, Kadin JA, Kaloff C, Kennedy K, Koutsourakis M, Lloyd KCK,
Marschall S, Mason J, McKerlie C, McLeod MP, von Melchner H, Moore M,
Mujica AO, Nagy A, Nefedov M, Nutter LM, Pavlovic G, Peterson JL,
Pollock J, Ramirez-Solis R, Rancourt DE, Raspa M, Remacle JE, Ringwald
M, Rosen B, Rosenthal N, Rossant J, Ruiz Noppinger P, Ryder E, Schick
JZ, Schnütgen F, Schofield P, Seisenberger C, Selloum M, Simpson EM,
Skarnes WC, Smedley D, Stanford WL, Stewart AF, Stone K, Swan K,
Tadepally H, Teboul L, Tocchini-Valentini GP, Valenzuela D, West AP,
Yamamura KI, Yoshinaga Y, Wurst W.
Mamm Genome. 2012 Oct;23(9-10):580-6. PMID:
22968824
DOI:
10.1007/s00335-012-9422-2
Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system.
Huang Y, Pettitt SJ, Guo G, Liu G, Li MA, Yang F, Bradley A.
Nucleic Acids Res. 2012 Feb 1;40(3):e21. PMID:
22127858
DOI:
10.1093/nar/gkr908
2011
High efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes.
Bird AW, Erler A, Fu J, Hériché JK, Maresca M, Zhang Y, Hyman AA, Stewart AF.
Nat Methods. 2011 Dec 4;9(1):103-9. PMID:
22138824
DOI:
10.1038/nmeth.1803
Disease model discovery and translation. Introduction.
Brown SD.
Mamm Genome. 2011 Aug;22(7-8):361. PMID:
21796466
DOI:
10.1007/s00335-011-9352-4
HIF-VEGF pathways are critical for chronic otitis media in Junbo and Jeff mouse mutants.
Cheeseman MT, Tyrer HE, Williams D, Hough TA, Pathak P, Romero MR,
Hilton H, Bali S, Parker A, Vizor L, Purnell T, Vowell K, Wells S,
Bhutta MF, Potter PK, Brown SD.
PLoS Genet. 2011 Oct;7(10):e1002336. PMID:
22028672
DOI:
10.1371/journal.pgen.1002336
Genetic screens using the piggyBac transposon.
Chew SK, Rad R, Futreal PA, Bradley A, Liu P.
Methods. 2011 Apr;53(4):366-71. PMID:
21185377
DOI:
10.1016/j.ymeth.2010.12.022
Recombineering BAC transgenes for protein tagging.
Ciotta G, Hofemeister H, Maresca M, Fu J, Sarov M, Anastassiadis K, Stewart AF.
Methods. 2011 Feb;53(2):113-9. PMID:
20868752
DOI:
10.1016/j.ymeth.2010.09.003
Disruption of mouse Slx4, a regulator of structure-specific nucleases, phenocopies Fanconi anemia.
Crossan GP, van der Weyden L, Rosado IV, Langevin F, Gaillard PH,
McIntyre RE; Sanger Mouse Genetics Project, Gallagher F, Kettunen MI,
Lewis DY, Brindle K, Arends MJ, Adams DJ, Patel KJ
Nat Genet. 2011 Feb;43(2):147-52. PMID:
21240276
DOI:
10.1038/ng.752
Recombination-mediated genetic engineering of large genomic DNA transgenes.
Ejsmont RK, Ahlfeld P, Pozniakovsky A, Stewart AF, Tomancak P, Sarov M.
Methods Mol Biol. 2011;772:445-58. PMID:
22065454
DOI:
10.1007/978-1-61779-228-1_26
Forward and reverse genetics through derivation of haploid mouse embryonic stem cells.
Elling U, Taubenschmid J, Wirnsberger G, O’Malley R, Demers SP,
Vanhaelen Q, Shukalyuk AI, Schmauss G, Schramek D, Schnuetgen F, von
Melchner H, Ecker JR, Stanford WL, Zuber J, Stark A, Penninger JM.
Cell Stem Cell. 2011 Dec 2;9(6):563-74. PMID:
22136931
DOI:
10.1016./j.stem.2011.10.012
Generating conditional knockout mice.
Friedel RH, Wurst W, Wefers B, Kühn R
Methods Mol Biol. 2011;693;205-31. PMID:
21080282
DOI:
10.1007/978-1-60761-974-1_12
Mouse phenotyping.
Fuchs H, Gailus-Durner V, Adler T, Aguilar-Pimentel JA, Becker L,
Calzada-Wack J, Da Silva-Buttkus P, Neff F, Götz A, Hans W, Hölter SM,
Horsch M, Kastenmüller G, Kemter E, Lengger C, Maier H, Matloka M,
Möller G, Naton B, Prehn C, Puk O, Rácz I, Rathkolb B, Römisch-Margl W,
Rozman J, Wang-Sattler R, Schrewe A, Stöger C, Tost M, Adamski J, Aigner
B, Beckers J, Behrendt H, Busch DH, Esposito I, Graw J, Illig T,
Ivandic B, Klingenspor M, Klopstock T, Kremmer E, Mempel M, Neschen S,
Ollert M, Schulz H, Suhre K, Wolf E, Wurst W, Zimmer A, Hrabé de Angelis
M.
Methods. 2011 Feb;53(2):120-35. Review. PMID:
20708688
DOI:
10.1016/j.ymeth.2010.08.006
High-throughput mouse phenotyping.
Gates H, Mallon AM, Brown SD; EUMODIC Consortium.
Methods. 2011 Apr;53(4):394-404. PMID:
21185382
DOI:
10.1016/j.ymeth.2010.12.017
BioMart Central Portal: an open database network for the biological community.
Guberman JM, Ai J, Arnaiz O, Baran J, Blake A, Baldock R, Chelala C,
Croft D, Cros A, Cutts RJ, Di Génova A, Forbes S, Fujisawa T, Gadaleta
E, Goodstein DM, Gundem G, Haggarty B, Haider S, Hall M, Harris T, Haw
R, Hu S, Hubbard S, Hsu J, Iyer V, Jones P, Katayama T, Kinsella R, Kong
L, Lawson D, Liang Y, Lopez-Bigas N, Luo J, Lush M, Mason J, Moreews F,
Ndegwa N, Oakley D, Perez-Llamas C, Primig M, Rivkin E, Rosanoff S,
Shepherd R, Simon R, Skarnes B, Smedley D, Sperling L, Spooner W,
Stevenson P, Stone K, Teague J, Wang J, Wang J, Whitty B, Wong DT,
Wong-Erasmus M, Yao L, Youens-Clark K, Yung C, Zhang J, Kasprzyk A.
Database (Oxford). 2011 Sep 18;2011:bar041. PMID:
21930507
DOI:
10.1093/database/bar041
Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis.
Hofemeister H, Ciotta G, Fu J, Seibert PM, Schulz A, Maresca M, Sarov M, Anastassiadis K, Stewart AF.
Methods. 2011 Apr;53(4):437-52. PMID:
21195765
DOI:
10.1016/j.ymeth.2010.12.026
A homozygous mutant embryonic stem cell bank applicable for phenotype-driven genetic screening.
Horie K, Kokubu C, Yoshida J, Akagi K, Isotani A, Oshitani A, Yusa K, Ikeda R, Huang Y, Bradley A, Takeda J.
Nat Methods. 2011 Oct 23;8(12):1071-7. PMID:
22020066
DOI:
10.1038/nmeth.1739
Technical approaches for mouse models of human disease.
Justice MJ, Siracusa LD, Stewart AF.
Dis Model Mech. 2011 May;4(3):305-10. Review. PMID:
21558063
DOI:
10.1242/dmm.000901
Conditional RNAi in mice.
Kleinhammer A, Deussing J, Wurst W, Kühn R.
Methods. 2011 Feb;53(2):142-50. Review. PMID:
20705138
DOI:
10.1016/j.ymeth.2010.08.003
Constitutive and conditional RNAi transgenesis in mice.
Kleinhammer A, Wurst W, Kühn R.
Methods. 2011 Apr;53(4):430-6. PMID:
21184828
DOI:
10.1016/j.ymeth.2010.12.015
Animal models for arthritis: innovative tools for prevention and treatment.
Kollias G, Papadaki P, Apparailly F, Vervoordeldonk MJ, Holmdahl R,
Baumans V, Desaintes C, Di Santo J, Distler J, Garside P, Hegen M,
Huizinga TW, Jüngel A, Klareskog L, McInnes I, Ragoussis I, Schett G,
Hart B, Tak PP, Toes R, van den Berg W, Wurst W, Gay S.
Ann Rheum Dis. 2011 Aug;70(8):1357-62. Review. PMID:
21628308
DOI:
10.1136/ard.2010.148551
Fancd2 counteracts the toxic effects of naturally produced aldehydes in mice.
Langevin F, Crossan GP, Rosado IV, Arends MJ, Patel KJ.
Nature. 2011 Jul 6;475(7354):53-8. PMID:
21734703
DOI:
10.1038/nature10192
Crafting rat genomes with zinc fingers.
Li MA, Bradley A
Nat Biotechnol. 2011;29;39-41. PMID:
21221099
DOI:
10.1038/nbt.1749
Mobilization of giant piggyBac transposons in the mouse genome.
Li MA, Turner DJ, Ning Z, Yusa K, Liang Q, Eckert S, Rad L, Fitzgerald TW, Craig NL, Bradley A.
Nucleic Acids Res. 2011 Dec;39(22):e148. PMID:
21948799
DOI:
10.1093/nar/gkr764
Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development.
Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani H, Tessier-Lavigne M, Wurst W, Friedel RH.
Mol Cell Neurosci. 2011 Feb;46(2):419-31. PMID:
21122816
DOI:
10.1016/j.mcn.2010.11.005
Methods for extracting function from mammalian genomes.
von Melchner H, Stewart AF.
Methods. 2011 Apr;53(4):329-30. PMID:
21392581
DOI:
10.1016/j.ymeth.2011.03.002
Aspartoacylase-LacZ Knockin Mice: An Engineered Model of Canavan Disease.
Mersmann N, Tkachev D, Jelinek R, Röth PT, Möbius W, Ruhwedel T, Rühle S, Weber-Fahr W, Sartorius A, Klugmann M
PLoS One. 2011;6(5):e20336. PMID:
21625469
DOI:
10.1371/journal.pone.0020336
Cop1 constitutively regulates c-Jun protein stability and functions as a tumor suppressor in mice.
Migliorini D, Bogaerts S, Defever D, Vyas R, Denecker G, Radaelli E, Zwolinska A, Depaepe V, Hochepied T, Skarnes WC, Marine JC
J Clin Invest. 2011 Apr 1;121(4):1329-43. PMID:
21403399
DOI:
10.1172/JCI45784
Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting.
Nedelkova M, Maresca M, Fu J, Rostovskaya M, Chenna R, Thiede C, Anastassiadis K, Sarov M, Stewart AF.
Nucleic Acids Res. 2011 Nov 1;39(20):e137. PMID:
21852329
DOI:
10.1093/nar/gkr668
BioMart as an integration solution for the International Knockout Mouse Consortium.
Oakley DJ, Iyer V, Skarnes WC, Smedley D.
Database (Oxford). 2011 Sep 18;2011:bar028. PMID:
21930503
DOI:
10.1093/database/bar028
Expression of the splicing factor gene SFRS10 is reduced in human obesity and contributes to enhanced lipogenesis.
Pihlajamäki J, Lerin C, Itkonen P, Boes T, Floss T, Schroeder J,
Dearie F, Crunkhorn S, Burak F, Jimenez-Chillaron JC, Kuulasmaa T,
Miettinen P, Park PJ, Nasser I, Zhao Z, Zhang Z, Xu Y, Wurst W, Ren H,
Morris AJ, Stamm S, Goldfine AB, Laakso M, Patti ME.
Cell Metab. 2011 Aug 3;14(2):208-18. PMID:
21803291
DOI:
10.1016/j.cmet.2011.06.007
A resource of vectors and ES cells for targeted deletion of microRNAs in mice.
Prosser HM, Koike-Yusa H, Cooper JD, Law FC, Bradley A.
Nat Biotechnol. 2011 Aug 7;29(9):840-5. PMID:
21822254
DOI:
10.1038/nbt.1929
Glutamatergic and dopaminergic neurons mediate anxiogenic and anxiolytic effects of CRHR1.
Refojo D, Schweizer M, Kuehne C, Ehrenberg S, Thoeringer C, Vogl AM,
Dedic N, Schumacher M, von Wolff G, Avrabos C, Touma C, Engblom D,
Schütz G, Nave KA, Eder M, Wotjak CT, Sillaber I, Holsboer F, Wurst W,
Deussing JM.
Science. 2011 Sep 30;333(6051):1903-7. PMID:
21885734
DOI:
10.1126/science.1202107
The IKMC web portal: a central point of entry to data and resources from the International Knockout Mouse Consortium.
Ringwald M, Iyer V, Mason JC, Stone KR, Tadepally HD, Kadin JA, Bult
CJ, Eppig JT, Oakley DJ, Briois S, Stupka E, Maselli V, Smedley D, Liu
S, Hansen J, Baldock R, Hicks GG, Skarnes WC
Nucleic Acids Res. 2011;39;D849-55. PMID:
20929875
DOI:
10.1093/nar/gkq879
Unraveling the genetics of otitis media: from mouse to human and back again.
Rye MS, Bhutta MF, Cheeseman MT, Burgner D, Blackwell JM, Brown SD, Jamieson SE.
Mamm Genome. 2011 Feb;22(1-2):66-82. PMID:
21107580
DOI:
10.1007/s00335-010-9295-1
Resources for proteomics in mouse embryonic stem cells.
Schnütgen F, Ehrmann F, Poser I, Hubner NC, Hansen J, Floss T, deVries I, Wurst W, Hyman A, Mann M, von Melchner H.
Nat Methods. 2011 Feb;8(2):103-4. PMID:
21278719
DOI:
10.1038/nmeth0211-103
High throughput gene trapping and postinsertional modifications of gene trap alleles.
Schnütgen F, Ehrmann F, Ruiz-Noppinger P, von Melchner H.
Methods. 2011 Apr;53(4):347-55. PMID:
21334922
DOI:
10.1016/j.ymeth.2010.12.037
A conditional knockout resource for the genome-wide study of mouse gene function.
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V,
Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu
J, Nefedov M, de Jong PJ, Stewart AF, Bradley A
Nature. 2011 Jun 15;474(7351):337-42. PMID:
21677750
DOI:
10.1038/nature10163
Cre recombinase resources for conditional mouse mutagenesis.
Smedley D, Salimova E, Rosenthal N.
Methods. 2011 Apr;53(4):411-6. PMID:
21195764
DOI:
10.1016/j.ymeth.2010.12.027
Utilization of a mutagenesis screen to generate mouse models of hyperaldosteronism.
Spyroglou A, Wagner S, Gomez-Sanchez C, Rathkolb B, Wolf E,
Manolopoulou J, Reincke M, Bidlingmaier M, Hrabé de Angelis M,
Beuschlein F.
Endocrinology. 2011 Jan;152(1):326-31. PMID:
21068155
DOI:
10.1210/en.2010-1081
Bi-allelic gene targeting in mouse embryonic stem cells.
Tate PH, Skarnes WC.
Methods. 2011 Apr;53(4):331-8. PMID:
21288739
DOI:
10.1016/j.ymeth.2010.12.025
Rapid and efficient reprogramming of somatic cells
to induced pluripotent stem cells by retinoic acid receptor gamma and
liver receptor homolog 1.
Wang W, Yang J, Liu H, Lu D, Chen X, Zenonos Z, Campos LS, Rad R, Guo G, Zhang S, Bradley A, Liu P.
Proc Natl Acad Sci U S A. 2011 Nov 8;108(45):18283-8. PMID:
21990348
DOI:
10.1073/pnas.1100893108
Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells.
Yusa K, Rashid ST, Strick-Marchand H, Varela I, Liu PQ, Paschon DE,
Miranda E, Ordóñez A, Hannan NR, Rouhani FJ, Darche S, Alexander G,
Marciniak SJ, Fusaki N, Hasegawa M, Holmes MC, Di Santo JP, Lomas DA,
Bradley A, Vallier L.
Nature. 2011 Oct 12;478(7369):391-4. PMID:
21993621
DOI:
10.1038/nature10424
A hyperactive piggyBac transposase for mammalian applications.
Yusa K, Zhou L, Li MA, Bradley A, Craig NL
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1531-6. PMID:
21205896
DOI:
10.1073/pnas.1008322108
2010
A practical summary of site-specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2.
Anastassiadis K, Glaser S, Kranz A, Berhardt K, Stewart AF
Methods Enzymol. 2010;477;109-23. PMID:
20699139
DOI:
10.1016/S0076-6879(10)77007-5
MouseBook: an integrated portal of mouse resources.
Blake A, Pickford K, Greenaway S, Thomas S, Pickard A, Williamson
CM, Adams NC, Walling A, Beck T, Fray M, Peters J, Weaver T, Brown SD,
Hancock JM, Mallon AM
Nucleic Acids Res. 2010;38;D593-9. PMID:
19854936
DOI:
10.1093/nar/gkp867
Phenotypic annotation of the mouse X chromosome.
Cox BJ, Vollmer M, Tamplin O, Lu M, Biechele S, Gertsenstein M, van Campenhout C, Floss T, Kühn R, Wurst W, Lickert H, Rossant J
Genome Res. 2010;20;1154-64. PMID:
20548051
DOI:
10.1101/gr.105106.110
ARCHAEA-ExPRESs targeting of alpha-tubulin 4 mRNA: a model for high-specificity trans-splicing.
Deidda G, Rossi N, Putti S, Tocchini-Valentini GP
FASEB J. 2010;24;2976-84. PMID:
20371619
DOI:
10.1096/fj.10-154658
A recombineering pipeline to make conditional targeting constructs.
Fu J, Teucher M, Anastassiadis K, Skarnes W, Stewart AF
Methods Enzymol. 2010;477;125-44. PMID:
20699140
DOI:
10.1016/S0076-6879(10)77008-7
Down syndrome: from understanding the neurobiology to therapy.
Gardiner K, Herault Y, Lott IT, Antonarakis SE, Reeves RH, Dierssen M
J Neurosci. 2010;30;14943-5. PMID:
21068296
DOI:
10.1523/JNEUROSCI.3728-10.2010
Gene knockdown in the mouse through RNAi.
Kleinhammer A, Wurst W, Kühn R
Methods Enzymol. 2010;477;387-414. PMID:
20699152
DOI:
10.1016/S0076-6879(10)77020-8
An improved Flp deleter mouse in C57Bl/6 based on Flpo recombinase.
Kranz A, Fu J, Duerschke K, Weidlich S, Naumann R, Stewart AF, Anastassiadis K
Genesis. 2010;48;512-20. PMID:
20506501
DOI:
10.1002/dvg.20641
Jarid2 is a PRC2 component in embryonic stem cells
required for multi-lineage differentiation and recruitment of PRC1 and
RNA Polymerase II to developmental regulators.
Landeira D, Sauer S, Poot R, Dvorkina M, Mazzarella L, Jørgensen HF,
Pereira CF, Leleu M, Piccolo FM, Spivakov M, Brookes E, Pombo A, Fisher
C, Skarnes WC, Snoek T, Bezstarosti K, Demmers J, Klose RJ, Casanova M,
Tavares L, Brockdorff N, Merkenschlager M, Fisher AG
Nat Cell Biol. 2010;12;618-24. PMID:
20473294
DOI:
10.1038/ncb2065
Genome-wide forward genetic screens in mouse ES cells.
Li MA, Pettitt SJ, Yusa K, Bradley A
Methods Enzymol. 2010;477;217-42. PMID:
20699144
DOI:
10.1016/S0076-6879(10)77012-9
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EuroPhenome: a repository for high-throughput mouse phenotyping data.
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Hans W, Horsch M, Rozman J, Calzada-Wack J, Kunder S, Naton B,
Gailus-Durner V, Fuchs H, Schulz H, Beckers J, Busch DH, Burbach JP,
Smidt MP, Quintanilla-Martinez L, Esposito I, Klopstock T, Klingenspor
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Research funding. Sustaining the data and bioresource commons.
Schofield PN, Eppig J, Huala E, de Angelis MH, Harvey M, Davidson D,
Weaver T, Brown S, Smedley D, Rosenthal N, Schughart K, Aidinis V,
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CIN85 regulates dopamine receptor endocytosis and governs behaviour in mice.
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Floxin, a resource for genetically engineering mouse ESCs.
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Nat Methods. 2010;7;50-2. PMID:
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Finding and sharing: new approaches to registries of databases and services for the biomedical sciences.
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Balling R, Birney E, Blake A, Bongcam-Rudloff E, Brookes AJ, Cesareni G,
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Einhorn D, Tocchini-Valentini G, Hrabe de Angelis M, Rosenthal N,
CASIMIR Rome Meeting participants
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Identification of protein/DNA contacts with dimethyl sulfate: methylation protection and methylation interference.
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piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.
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Novel caspase-suicide proteins for tamoxifen-inducible apoptosis.
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ES cell pluripotency and germ-layer formation require the SWI/SNF chromatin remodeling component BAF250a.
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Proc Natl Acad Sci U S A. 2008;105;6656-61. PMID:
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Proc Natl Acad Sci U S A. 2008;105;17453-6. PMID:
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MausDB: an open source application for phenotype data and mouse colony management in large-scale mouse phenotyping projects.
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BMC Bioinformatics. 2008;9;169. PMID:
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Deletion of glucose transporter GLUT8 in mice increases locomotor activity.
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Enhanced gene trapping in mouse embryonic stem cells.
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Nucleic Acids Res. 2008;36;e133. PMID:
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Simultaneous Cre-mediated conditional knockdown of two genes in mice.
Steuber-Buchberger P, Wurst W, Kühn R
Genesis. 2008;46;144-51. PMID:
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A DNA transposon-based approach to validate oncogenic mutations in the mouse.
Su Q, Prosser HM, Campos LS, Ortiz M, Nakamura T, Warren M, Dupuy AJ, Jenkins NA, Copeland NG, Bradley A, Liu P
Proc Natl Acad Sci U S A. 2008;105;19904-9. PMID:
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Chromosomal transposition of PiggyBac in mouse embryonic stem cells.
Wang W, Lin C, Lu D, Ning Z, Cox T, Melvin D, Wang X, Bradley A, Liu P
Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9290-5. PMID:
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2007
A mouse for all reasons.
International Mouse Knockout Consortium, Collins FS, Rossant J, Wurst W
Cell. 2007;128;9-13. PMID:
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A new partner for the international knockout mouse consortium.
Collins FS, Finnell RH, Rossant J, Wurst W
Cell. 2007;129;235. PMID:
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Hdac2 regulates the cardiac hypertrophic response by modulating Gsk3 beta activity.
Trivedi CM, Luo Y, Yin Z, Zhang M, Zhu W, Wang T, Floss T,
Goettlicher M, Noppinger PR, Wurst W, Ferrari VA, Abrams CS, Gruber PJ,
Epstein JA
Nat Med. 2007;13;324-31. PMID:
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The International Gene Trap Consortium Website: a portal to all publicly available gene trap cell lines in mouse.
Nord AS, Chang PJ, Conklin BR, Cox AV, Harper CA, Hicks GG, Huang
CC, Johns SJ, Kawamoto M, Liu S, Meng EC, Morris JH, Rossant J, Ruiz P,
Skarnes WC, Soriano P, Stanford WL, Stryke D, von Melchner H, Wurst W,
Yamamura K, Young SG, Babbitt PC, Ferrin TE
Nucleic Acids Res. 2006;34;D642-8. PMID:
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Engineering embryonic stem cells with recombinase systems.
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Methods Enzymol. 2006;420;100-36. PMID:
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EMPReSS: standardized phenotype screens for functional annotation of the mouse genome.
Brown SD, Chambon P, de Angelis MH,
Nat Genet. 2005;37;1155. PMID:
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Genomewide production of multipurpose alleles for the functional analysis of the mouse genome.
Schnütgen F, De-Zolt S, Van Sloun P, Hollatz M, Floss T, Hansen J,
Altschmied J, Seisenberger C, Ghyselinck NB, Ruiz P, Chambon P, Wurst W,
von Melchner H
Proc Natl Acad Sci U S A. 2005;102;7221-6. PMID:
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The knockout mouse project.
Austin CP, Battey JF, Bradley A, Bucan M, Capecchi M, Collins FS,
Dove WF, Duyk G, Dymecki S, Eppig JT, Grieder FB, Heintz N, Hicks G,
Insel TR, Joyner A, Koller BH, Lloyd KC, Magnuson T, Moore MW, Nagy A,
Pollock JD, Roses AD, Sands AT, Seed B, Skarnes WC, Snoddy J, Soriano P,
Stewart DJ, Stewart F, Stillman B, Varmus H, Varticovski L, Verma IM,
Vogt TF, von Melchner H, Witkowski J, Woychik RP, Wurst W, Yancopoulos
GD, Young SG, Zambrowicz B
Nat Genet. 2004;36;921-4. PMID:
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The European dimension for the mouse genome mutagenesis program.
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Berns A, Bradley A, Brown S, Carmeliet P, Chambon P, Cox R, Davidson D,
Davies K, Duboule D, Forejt J, Granucci F, Hastie N, de Angelis MH,
Jackson I, Kioussis D, Kollias G, Lathrop M, Lendahl U, Malumbres M, von
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A public gene trap resource for mouse functional genomics.
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A large-scale, gene-driven mutagenesis approach for the functional analysis of the mouse genome.
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Functional analysis of secreted and transmembrane proteins critical to mouse development.
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An LDL-receptor-related protein mediates Wnt signalling in mice.
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Rapid sequence analysis of gene trap integrations to generate a resource of insertional mutations in mice.
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